高生物相容性奈米材料應用在金屬離子 及三聚氰胺檢測

Detection of Melamine and Metallic Ions Based on Biocompatible Nanomaterials

唐婕(Maria Jessabel Talite)

指導教授 ： 院繼祖

中原大學/理學院/物理研究所/碩士(2016年)

https://doi.org/10.6840/cycu201600714

摘要

金和銀奈米團等發光材料由於擁有良好的光物理性質以及能廣泛應用於生物性探測上等原因，目前受到了很大的關注且成為有良好敏感度測試的物質之一。除了有很強的光學性質之外，金和銀等奈米團也擁有很好的生物相容性，也能簡單的合成。為了保護環境以及我們的身體，我認為研究出快速、低成本、簡單且高敏感度的水溶液重金屬探測法，並且應用於測量三聚氰胺及重金屬，是必要且不容忽視的。

我的研究工作是要找出重金屬粒子對於生物相容性奈米材料的光強度及生命期的反應，並利用這些反應來檢測三聚氰胺及金屬粒子。結果顯示，當我們增加金和銀等生物相容性的奈米團的濃度時，發光光譜以及生命期會相對地下降及變短。數據結果顯示，三聚氰胺和Fe3+, Cu2+, Hg2+, Zn2+, Na+等金屬粒子的濃度增加時，奈米材料的生命期是相對下降的。當我們加入不同濃度的三聚氰胺和Fe3+, Cu2+, Hg2+, Zn2+, Na+等金屬粒子時可以測量到發光光譜。發光強度及生命期的降低同時也是我們可以清楚分辨且看到的。

關鍵字

金奈米群集 ； 銀奈米群集 ； 生物相容性奈米材料 ； 三聚氫胺 ； 金屬離子 ； 熒光強度 ； 熒光生命週期

並列摘要

Fluorescent nanomaterials such as Gold (Au) and Silver (Ag) nanoclusters in liquid phase have become one of the most essential nanomaterial and have aroused much attention in research due to their excellent photophysical properties and wide range of applications as optical label for biosensing. In addition to their outstanding optical properties, Au and Ag Nanoclusters have the desired benefits of biocompatibility and simple synthetic routes. The study looked into the development of novel heavy metals detection methods that are rapid, low-cost, simple, highly sensitive, and applicable to aqueous systems for the detection of melamine and metal ions in view of the fact that it has become an urgent need to protect our environment and health. The research work seeks to investigate the effects of heavy metal ions on fluorescence intensity and fluorescence lifetime of the biocompatible nanomaterials and explore the use of this ineraction as sensors for melamine and metal ions.
The results showed that upon addition and gradual increase in the volume and concentration of fluorescent indicator to solution-processed biocompatible nanomaterials such as Gold and Silver Nanoclusters, fluorescence intensity decreases and the intact span of fluorescence lifetime shortens. It was found that as the concentration of melamine and selected metal ions such as Fe3+, Cu2+, Hg2+, Zn2+, Na+ metal ion increases, the fluorescence lifetime of nanomaterial decreases. Fluorescence spectra were recorded and fluorescence responses were evaluated upon addition of different concentrations of quenchers. Clearly, a distinguishable decrease in the fluorescence intensity and fluorescence lifetime can be observed.

並列關鍵字

gold nanoclusters ； silver nanoclusters ； biocompatible nanomaterials ； melamine ； metal ions ； fluorescence intensity ； fluorescence lifetime

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