以聚甲基丙烯酸烷基酯-二乙烯苯整體成形管柱微萃取結合微乳化電層析分析非類固醇抗發炎藥物

Analyses of non-steroidal anti-inflammatory drugs by poly(methacrylate-divinylbenzene) monolith microextraction coupled with microemulsion electrokinetic chromatographic separation

吳令台(Lin-Tai Wu)

指導教授 ： 黃悉雅

中原大學/理學院/化學研究所/碩士(2013年)

https://doi.org/10.6840/cycu201301125

摘要

本篇論文主要為開發新形態高分子整體成形管柱微萃取(polymer monolith microextraction, PMME)之萃取管柱材料：結合甲基丙烯酸烷基酯單體與二乙烯苯交聯劑並以乙二醇做為擴孔劑，針對水樣中九種非類固醇抗發炎藥物(non-steroid anti-inflammatory durgs, NSAIDs)進行萃取，最後以毛細管微乳化方法檢測其萃取效果。
實驗首先以聚(甲基丙烯酸十八烷基酯-二乙烯苯) (poly(SMA-DVB))整體成形毛細管柱作為萃取管柱，並對活化溶劑的種類及流速、清洗溶劑的種類及流速、萃取與脫附溶劑的流速、萃取管柱中擴孔劑PEG之添加量、萃取管柱長度、高分子材料聚合溶液的單體比例進行最佳化；之後再以不同碳鏈長度之甲基丙烯酸烷基酯類單體合成萃取管柱，藉以不同碳鏈長度來探討不同親疏水性對萃取的影響，最後得到以聚(甲基丙烯酸八烷基酯-二乙烯苯)之整體成形萃取管柱有最佳萃取效能，其回收率達到73.8~99.6 %，以相同管柱重覆萃取之標準偏差(RSD of run-to-run)小於10.7 %，不同批次製備管柱之標準偏差(RSD of column-to-column)小於13.5 %。

關鍵字

整體成形管柱微萃取 ； 整體成形毛細管柱 ； 微乳化電層析 ； 非類固醇抗發炎藥物

並列摘要

In this study, we developed a new monolithic material for polymer monolith microextraction (PMME) that was prepared in the capillary and applied to extract non-steroid anti-inflammatory durgs (NSAIDs) in water solution. Herein microemulsion electrokinetic chromatography (MEEKC) was used to check the extraction efficiency.
At first, poly(stearyl methacrylate-divinylbenzene) (poly(SMA-DVB)) monolithic column was employed, and the extracted parameters were optimized that included solvent type, flow rate, column length and composition of extraction column. Then, different alkyl chain length of alkyl methacrylate monomers have been tested and different extracted efficiency was obtained due to their hydrophobic and hydrophilic interaction. Finally poly(lauryl methacrylate- divinylbenzene) (poly(LMA-DVB)) monolithic column was the best PMME material which has higher extraction efficiency (recovery from 73.8% to 99.6%) and lower RSD% (run to run: < 10.7 %, column to column: < 13.5 %).

並列關鍵字

microemulsion electrokinetic chromatography ； monolithic capillary ； polymer monolith microextraction ； non-steroid anti-inflammatory durgs

參考文獻

Abdel-Rehim, M. (2004). New trend in sample preparation: on-line microextraction in packed syringe for liquid and gas chromatography applications: I. Determination of local anaesthetics in human plasma samples using gas chromatography–mass spectrometry. Journal of Chromatography B, 801(2), 317-321.

Agüera, A., Perez Estrada, L. A., Ferrer, I., Thurman, E. M., Malato, S., & Fernández‐Alba, A. R. (2005). Application of time‐of‐flight mass spectrometry to the analysis of phototransformation products of diclofenac in water under natural sunlight. Journal of mass spectrometry, 40(7), 908-915.

Aguilar-Arteaga, K., Rodriguez, J. A., Miranda, J. M., Medina, J., & Barrado, E. (2010). Determination of non-steroidal anti-inflammatory drugs in wastewaters by magnetic matrix solid phase dispersion–HPLC. Talanta, 80(3), 1152-1157.

Araujo, L., Wild, J., Villa, N., Camargo, N., Cubillan, D., & Prieto, A. (2008). Determination of anti-inflammatory drugs in water samples, by in situ derivatization, solid phase microextraction and gas chromatography–mass spectrometry. Talanta, 75(1), 111-115.

Arthur, C. L., & Pawliszyn, J. (1990). Solid phase microextraction with thermal desorption using fused silica optical fibers. Analytical chemistry, 62(19), 2145-2148.