金屬基材之表面自組裝雙離子化修飾與其抗生物沾黏性質控制

金屬材料被廣泛的應用在醫療器材/零件上，其醫療用級鈦金屬與不鏽鋼為大宗，然而雖然具有良好的機械性質及加工性質，但生物相容性的不足，使得在應用於人體各部分的補強與修復時，往往造成人體的免疫排斥反應的發生，造成人體二次傷害(例：骨不鏽鋼釘於骨頭復元時因生物相容性低，人體免疫反應發生時組織將其視為外來物質，對其進行包覆造成傷癒必須將其切除)或者是無法長時間使用於人體(例：鈦金屬心血管支架因血液相容性不佳，在一定時間使用下血液中蛋白質貼附，漸而吸引其他血球貼附其上最終失效)，本次研究的重點為透過自組裝雙離子化修飾金屬表面，來達到生物沾黏的控制，選擇了與人體具有高度相容性的單體結構-雙離子性硫代甜菜鹼(SBMA)與具有特定反應性的單體結構-甲基丙烯酸縮水甘油酯(GMA)，進行共聚三嵌段高分子(pSBMAn-b-pGMAn -b-pSBMAn ; n=25, 50, 75, 100)的製備合成，並針對鈦金屬與不鏽鋼利用熱誘導方式進行自組裝雙離子化修飾，且探討雙離子高分子鏈段(pSBMA)與提供基材表面固定化之高分子鏈段(pGMA)，在不同鏈段長度與比例下對於生物沾黏控制效果與表面接枝程度的影響。此研究使用核磁共振儀驗證所製備的雙離子共聚高分子其化學結構之正確性，並以水接觸角、橢圓儀、以及場效發射式掃描電子顯微鏡(FE-SEM)檢測其自組裝雙離子化基材表面物理與化學結構的特性。此研究以血漿蛋白、各式血球、細胞及細菌貼附實驗來探討自組裝雙離子化基材表面之抗生物分子沾黏效果，研究結果顯示此一界面結構設計確實能有效降低生物分子貼附行為發生，此研究工作更進一步改質不鏽鋼刀以及鈦金屬製牙根來實際應用於細菌貼附測試，其效果展現出良好的抗菌性質。

關鍵字

抗沾黏；自組裝；雙離子化；聚甲基縮水甘油脂；熱誘導；生物相容性

並列摘要

Metal materials are widely used in medical device or parts, and the most common were medical grade titanium and stainless steel. Although metals have good mechanical properties and processing properties but lack biocompatibility when applied to human body, such as the reinforcement or repair, usually occurring of the foreign body granuloma, caused injured again. This study focuses on the self-assembling zwitterionization via thermal-induced immobilization method for metal substrates to achieve the general biofouling control. We use the zwitterionic sulfobetaine monomer (SBMA) and glycidyl methacrylate monomer (GMA) to synthesize a set of triblock copolymers (pSBMAn-b-pGMAn-b-pSBMAn; n = 25, 50, 75, 100) with controlled block ratios of SBMA/GMA. The study also discussed the effect of copolymer grafting density on its antifouling capability. The outstanding antifouling performance of zwitterionic pSBMA brushes within stable chemisorption via covalent bounding can be achieved by epoxy groups of polyGMA reacted with hydroxyl groups on surface, which was determined by 1H NMR. The results showed that stainless steel surfaces anchored with pSBMA brushes provide good fouling resistance in plasma proteins, blood cells, tissue cells and general bacterial. The results also showed the modification method successfully applied to the antifouing coating on surgical stainless steel blade and titanium metal root for the control of bacterial resistance.

並列關鍵字

Anti-fouling；metal；self-assembled；zwitterionization；polyGMA；thermal induce；biocompatibility

參考文獻

[1] Ratner, B. D., Biomaterials Science: An Introduction to Materials in

[2] Ratner, B. D., Biomaterials science : an introduction to materials in medicine. Academic Press: San Diego, 1996

[3] Castner, D.G., Ratner, B.D., Biomedical surface science: Foundations to frontiers. Surf. Sci. 500 (2002) 28.

[5] Seeram Ramakrishna, Murugan Ramalingam, T.S Sampath Kumar, Winston O.Soboyejo, Biomaterials: A nano approach CRC Press, 2010

[6] Temenoff, J. S.; Mikos, A. G., Biomaterials:The Intersection of Biology and Materials Science. Pearson Education Hall: 2008; p 10.

國際替代計量

金屬基材之表面自組裝雙離子化修飾與其抗生物沾黏性質控制

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