酸鹼與溫度應答磁性微胞運用為藥物載體之研究

Study of pH- and temperature- responsive magnetic micelles gelatin-g-poly(NIPAAm-co-DMAAm-co-UA)-g-dextran/Fe3O4 as drug carrier

蘇昭名(Chao-Ming Su)

指導教授 ： 葛宗融

中原大學/工學院/生物醫學工程研究所/博士(2017年)

https://doi.org/10.6840/cycu201700754

摘要

酸鹼應答磁性微胞具備pH專一性與磁引導特性，可藉由此兩種特性增加特定環境的藥物釋放，並降低生物組織的副作用。本研究以明膠接枝聚(異丙基丙烯醯胺-二甲基丙烯酰胺-十一烯酸)接枝葡聚醣四氧化三鐵 (gelatin-g-poly(NIPAAm-co-DMAAm-co-UA)-g-dextran/Fe3O4，GPDF)，搭載藥物作為藥物載體進行藥物於特定環境釋放之研究。以超導量子干涉磁量儀(superconducting quantum interference device，SQUID)、傅利葉轉換紅外光譜(Fourier transform infrared spectroscopy，FTIR)與核磁共振光譜學(nuclear magnetic resonance spectroscopy，NMR)驗證磁性微胞之接枝與超順磁特性，運用此特性標定微胞，藉由磁性操控微胞移動於特定區域作藥物釋放。微胞於病變組織pH 6.6環境中較正常組織pH 7.2環境能提升近3倍的藥物釋放。本研究顯示GPDF在磁性標靶治療與藥物載體上具有相當的潛力。

關鍵字

磁性奈米粒子 ； 酸鹼應答磁性微胞 ； 低臨界溶液溫度

並列摘要

pH-responsive magnetic micelles are beneficial for time-controlled and site-specific drug deliver without triggering potential biological side effects. In this study, gelatin-g-poly(NIPAAm-co-DMAAm-co-UA)-g-dextran/Fe3O4 (GPDF) pH-responsive magnetic micelles have been synthesized to carry hydrophilic insulin-promoting factor, nicotinamide. Superconducting quantum interference device (SQUID), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR) analysis confirmed the grafting and superparamagnetic property of GP micelles. Use the magnetic property, manipulate the GP micelles to specific region for drug delivery. The amount of accumulative nicotinamide release in pH 6.6 was 3-fold higher than that in pH 7.2 suggesting GPDF is beneficial for targeting pathological regions. The present study demonstrated that GPDF is a potential drug carrier for pH and magnetic target therapy.

並列關鍵字

Magnetic nanoparticles ； pH responsive magnetic micelles ； lower critical solution temperature (LCST)

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