- 學位論文
細懸浮微粒對小鼠胚胎發育與大鼠子代發育之影響
Fine Particulate Matter Cause Rodent Embryonic and Developmental Toxicity
摘要
空氣汙染隨著科技的進步也隨著嚴重，此議題也在近年受到了重視，空氣汙染物中的懸浮微粒以碳為核心吸附著有機物質、重金屬等物質，其中粒徑大小小於2.5 μm稱之為細懸浮微粒（fine particulate matter），細懸浮微粒可隨著呼吸作用到達支氣管，通過血管進入人體經由血液循環影響到全身。當空氣汙染物隨著呼吸進入孕婦體內亦會影響到下一代，研究統計指出孕期間暴露於細懸浮微粒中出生嬰兒可能出現先天性心臟疾病、流產、早產、出生體重過低等狀況，且子代的中樞神經系統也可能因母體懷孕期間暴露於細懸浮微粒而受到影響。 為探討細懸浮微粒對胚胎與子代發育之影響，本實驗從胎兒著床前期開始進行探討，利用小鼠囊胚（Blastocyst）與細懸浮微粒共培養探討細懸浮微粒對於囊胚的細胞毒性，實驗結果發現細懸浮微粒會造成小鼠囊胚細胞數減少，以及體外培養胚胎著床與發育會隨著暴露劑量越高，著床狀況越低，在凋亡與壞死試驗中可見囊胚受損傷細胞可能在內細胞群。以小鼠進行體內試驗探討囊胚發育中可見暴露細懸浮微粒後發育到囊胚期的比率下降。接著探討出生後子代學習記憶能力及神經是否受到影響，使用孕期暴露於細懸浮微粒的大鼠出生後子代進行行為學試驗、腦部組織分析，可發現記憶能力、空間認知與神經型態皆因母體暴露細懸浮微粒後而有損傷的情形。
並列摘要
Air Pollution increases as progress of technology, and became a popular issue in recent years. The fine particulate matter is one of air pollution, which is carbon as the core and adsorption of organic matter, heavy metals and other substances, and particle size is less than 2.5 μm. Fine particulate matter can into our body by respiration, and it reach the bronchi, than translocate to blood vessel and then affect whole body through the blood circulation system. When pregnant women inhaled air, the offspring will also be affected. It has been report that exposed to fine particulate matter during pregnancy, new born may have congenital heart disease, miscarriage, premature birth, low birth weight, and central nervous system may also be affected. In order to know the effect of fine particulate matter on the development of the offspring, this study began from the preimplantation stage. To know the embryonic and developmental toxicity cause by fine particulate matters. We use blastocysts co-culture with fine particulate matters and intratracheal instillation in mice and rats to assess embryonic development and offspring development. Results show that fine particulate matters decreased the cell number of mouse blastocysts, increased apoptotic cell in blastocysts, and cause low implantation rate. Further, offspring that maternal fine particulate matter exposure, has learning and memory ability decreased, and injury neuron morphology.
參考文獻
延伸閱讀
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