- 學位論文
開發大氣電漿滅菌盒應用於細菌滅活之影響
Development of Atmospheric Pressure Cold Plasma Sterilization Box Applied to Inactivation Bacteria Impact
摘要
現今院內感染案件有越來越多的趨勢，主要感染方式可能是經由黏附細菌的醫療器材與病人交叉感染，或是經由接觸、媒介物等傳染途徑感染。然而針對降低院內感染的改善方式是將醫療器材有效地滅菌，但市面上的滅菌方式會造成滅菌時間較長、材料選用有限、使用時須為真空狀態等缺點，而大氣低溫電漿現已慢慢崛起也被廣泛應用於滅菌。本研究使用介電質阻擋放電（dielectric barrier discharge ,DBD）型式設計了低溫大氣電漿系統應用於微生物滅菌，所開發之低溫大氣電漿滅菌盒為體積小、成本低、可快速滅菌並應用於各式材料等優點。使用工作電壓為8.24 kV、總功率為27 W，利用光學發射光譜儀(Optical Emission Spectroscopy; OES)檢測到電漿中具有單態氧和OH自由基等活性氧物種，對大腸桿菌(Escherichia coli, E. coli)、金黃色葡萄球菌(Staphylococcus aureus, S.A.)進行不同時間的滅菌測試，滅菌率儼然達到99 %。因此證明本研究所開發之電漿系統視為可在短時間內達到滅菌之效果，希望本研究在未來可以商品化並且能提供醫院一個體積小、滅菌時間短、對於高分子材料亦能進行滅菌的電漿滅菌系統。
並列摘要
The cases of infection in hospital are increasing recently. The main pathway of infection are: the adhesion of bacterial on medical equipment, patient infection or cross contact vehicle of transmission. Sterilization can reduce the case of infection hospital, but most of way to sterilize may take a lot of time, materials are also limited and must in vacuum state, these cause the medical equipment can’t be used immediately and are inconvenient. The low temperature atmosphere plasma is gradually applied to sterilize. In this study, a new type of atmospheric plasma system with a Dielectric barrier Discharge (DBD) is designed, in order to sterilize the medical equipment in a short time and can be repeatable to use. After sterilization in different times, it seems to have reached 99% of sterilization efficiency to Escherichia coli and Staphylococcus aureus, the operating voltage is 8.24 kV, and total power is 27 W when activating, reactive oxygen species such as oxygen and hydroxyl radicals can be detected by Optical Emission Spectroscopy. The low temperature atmospheric plasma sterilization box I develop in this research is in a small size, low cost, simple to operate. Therefore, this study prove that this plasma system is consider to sterilization effectively in a short time. I hope this study can be commercialize in the future and can provide to hospital a plasma system that is in a small size, sterilize in a short time and polymer materials also can be sterilized by plasma system.