- 學位論文
以毛細管電泳檢測微量之菸鹼與非類固醇抗炎藥物
Determination of Trace-level Tobacco Alkaloids and Non-steroidal Anti-inflammatory Drugs by Capillary Electrophoresis
摘要
本論文第一部分將以毛細管微乳化電層析(microemulsion electrokinetic chromatrography, MMEKC)結合正常注射及陰離子選擇注射進行偵測十種非類固醇抗炎藥物(nonsteroid anti-inflammatory drugs, NSAIDs) 。最佳化背景電解液組成為pH 2磷酸溶液，包含3.31% 十二烷基磺酸鈉鹽，1.01% PGDA，8.61%異丙醇，15%乙腈(正常模式時)及13%乙腈(堆積模式時) 。當在堆積水段添加15%乙腈，及樣品基質中溶液添加10%乙腈進行陰離子選擇注射時，偵測極限可降低至0.033 ~ 0.297ng/mL。此微乳化電層析技術在正常注射模式時，能有效檢測出市售藥品和藥物貼片之使用前後殘留NSAIDs的濃度差異，而在陰離子選擇注射模式下，能有效檢測出環境水樣微量ibuprofen的殘留。 毛細管電泳具有高分離效率及低溶劑使用量的特性，而離子阱(ion trap)質譜儀則具有分子結構鑑定及快速檢測功能，本研究第二部分利用非水相毛細管電泳串聯電噴灑離子阱質譜儀(nonaqueous capillary electrophoresis mass spectrometry, NACE-MS)偵測六種菸草中之常見菸鹼(tobacco alkaloids)。最佳化之非水相背景電解液組成為40mM甲酸銨溶於30% 乙腈及70% 甲醇 (pH 4)。配合增加電壓進樣時間以提升菸鹼訊號的強度，偵測極限可降為0.41~4.45 ng/mL。可成功利用此簡單快速方法檢測出吸菸者吐出之二手菸殘留之菸鹼。
並列摘要
In the first part of the thesis, microemulsion electorkinetic chromatography (MEEKC) coupled normal injection and anion-selective exhaustive injection sweeping technology with MEEKC (ASEI-sweeping- MEEKC) were used to detect and analyze ten nonsteroid anti-inflammatory drugs (NSAIDs). The optimum background electrolyte was phosphoric acid (pH 2), which contained 3.31% SDS, 1.01% PGDA, 8.61% 2-propanol, 15% ACN (normal injection) or 13% ACN (ASEI-sweeping-MEEKC). ASEI sweeping technology with MEEKC which added 15% ACN in H2O plug and 10% ACN in sample matrix. The detection limits of ten NSAIDs were improved to the range of 0.033 ~ 0.297 ng/mL. The MEEKC coupled normal injection method was also applied in the tablets and comparing the commercial patches of the discrepancy concentration which used and unused. And the ASEI-sweeping-MEEKC has succeeded on detecting trace-level ibuprofen in the environment of the water samples. In the second part of the thesis, a nonaqueous capillary electrophoresis coupled to electrospray ionization-ion trap-mass spectrometry (NACE/ESI-MS) was used to determine six tobacco alkaloids. The optimum background electrolyte was composed of 40 mM FA (pH 4), 30% ACN and 70% methanol (v/v). Increasing injection time at high voltage could enhance the signals of tobacco alkaloid. The optimal conditions obtain the detection limits of tobacco alkaloids were improved to the range of 0.41 ~ 4.45 ng/mL. Finally, this technique has been succeeded in detecting trace-level tobacco alkaloids from secondhand smoke.