薄膜側向流動偵測條件最適化與禽流感病毒N2序列之偵測

The condition optimization of membrane based lateral-flow detection and detection of Avian Influenza N2 virus

簡瑞男(Jui-Nan Chien)

指導教授 ： 吳瑞璋

中原大學/工學院/化學工程研究所/碩士(2009年)

https://doi.org/10.6840/cycu200901085

摘要

禽流感(Avian Influenza)為家禽類的病毒性流行性感冒，通常是由流感病毒引起家禽類的一種從呼吸系統到嚴重全身敗血症等多種症狀的傳染病。禽流感病毒在基因上通常和人類流感病毒有區隔，如今可能出現的危機是由於該類病毒極易產生基因轉移現象，若經過突變再感染人類後，則可能會演變成人傳人的大流行，在人類體內還沒有被引發出任何抗體情況下，預估將造成極重大疫情。
本實驗以台灣地區所驗出的禽流感病毒H5N2型病毒株N2為基礎，並以N2病毒株中的DNA序列來進行免疫層析試紙分析法
(immunochromatography)測試，藉由改變緩衝溶液的濃度、薄膜的選擇、測試線的位置及增加電極伏特來達到訊號最佳及明顯化。而在偽訊號處理與驗證上，本研究將探討在進行薄膜分析時，所印製probe測試線的序列T連續性，與其所產生偽訊號的問題。

關鍵字

側向流動偵測 ； 禽流感病毒

並列摘要

Avian influenza refers to influenza caused by avian viruses, which is the infectious disease with many symptoms of respiratory system and body septicemia. The genes of avian influenza viruses are usually
different from those of human infected viruses. At present, some crisesoccurs because the viruses of avian influenza are extremely easy to
generate the gene mutation. If viruses have preceded mutation and
infected human, the situation will become more epidemic among people.
This study’s target, N2 strains, was selected as type H5N2 of avian Influenza viruses which had been identified in Taiwan areas. The genesequence of N2 strains was tested by the immunochromatographymethods. To achieve the best signal, the concentration of buffer solution, type of membrane, and position of test line, and electrode volt were
tested. This study also discussed the function of sequence T on theprinted test line and the problems of false signal generation during themembrane analysis.

並列關鍵字

immunochromatography ； lateral-flow

參考文獻

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