超音波促進經皮式藥物釋放效能的
可行性探討

中文摘要近年來，經皮遞藥系統快速發展，過去幾年許多文獻顯示超音波導入療法可有效克服皮膚障蔽提升經皮式藥物釋放效能。本研究嘗試以不同的超音波參數促進藥物進入皮膚組織的擴散速率。體外藥物釋放實驗系統中以新鮮豬皮模擬生物系統之擴散膜，擴散膜兩側有給藥池、及接受池，分別在擴散藥物端置放1 wt%葡萄糖水溶液，在接收端置放去離子水。探討不同參數的超音波所誘發的生物效應對於經皮式藥物釋放的效能影響；藉由調變不同的超音波參數，不同頻率(1 MHz和3 MHz)，強度由低至高(0.1-1.0 W/cm2)超音波刺激豬皮。另外，固定超音波輸出功率在0.5和1.0 W/cm2時，分別調整超音波工作週期比例為10%、20%與100%，實驗進行一小時後從接收端取樣，以酚-硫酸法量測接收端濃度。實驗最後使用傅立葉紅外線光譜儀(FTIR)分析藥物是否殘留於皮膚。實驗結果顯示，擴散濃度會隨著強度的增加而上升，實驗效能最佳之超音波組的擴散濃度較控制組高出約3倍；強度遞增伴隨溫度也呈非線性變化，因此於實驗過後，須進行熱效應的實驗校正。當超音波在相同平均功率時，連續波的刺激模式會使葡萄糖溶液擴散速率較快，藥物擴散濃度也隨不同的釋放時間點增長會逐漸遞增。本研究證實超音波的導入，可有效改善經皮遞藥的效能。

關鍵字

超音波；經皮；藥物釋放

並列摘要

Rescently, transdermal drug delivery system (TDDS) was developed quickly. There were many researches to reveal that sonophoresis can be an enhancer to promote the efficacy of transdermal drug delivery and disorder stratum corneum. This study tried to use different ultrasound parameters to promote the rate of drug permeability into skin tissue. In vitro study, the experiments were performed from fresh porcine skins as diffusion membranes. The porcine skin were arranged to separate the donor compartment from the receiver compartment. The donor compartment was initially filled with 1 wt% glucose solution and that the receiver compartment was filled with de-ionized water. The experiment included two groups. The first group is for the control and the second group was managed to employ applications of ultrasound as the ultrasound group. In both frequencies, 1 MHz and 3MHz, the ultrasound groups were set a rage of intensities at 0.1 to 1.0 W/cm2. Other types of ultrasound group were set the same output intensity and adjusted different duty cycles. After an hour, to analyze the concentrations from receiver compartment by phenol-sulfate acid method. Finally, Fourier transform infrared rays spectrometer (FTIR) detect whether the drug get into skin. Results showed that ultrasound applicated to the skin with the energy increase, and more glucose concentrations can be received. The best results of ultrasound group increased 3-fold. Due to high energies, the temperatures of donor compartment also increased nonlinearly. Hence, the experiment had to be calibrated thermal effect. When at the same ultrasound power, continuous wave exposure model can cause glucose solution diffuse fast. Diffuse concentratios as well as setting delivery time were dependent. The longer delivery time, the more concentrations can be received. The experiment has be demonstrated that ultrasound could promote transdermal drug delivery.

並列關鍵字

drug delivry ； ultrasound ； transdermal

參考文獻

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[8]Mitragotri S, and Kost J. (2000). Low-Frequency Sonophoresis: A Noninvasive Method of Drug Delivery and Diagnostics, Biotechnol Prog, 16, 488-492.

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超音波促進經皮式藥物釋放效能的可行性探討

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