探討大黃素對於小鼠囊胚死亡訊息與胚胎發育 之影響

Study of Cell Death Signaling and Embryonic Development in Emodin-treated Mouse Blastocysts

張美惠(Mei-Hui Chang)

指導教授 ： 詹文雄

中原大學/工學院/生物科技研究所/碩士(2009年)

https://doi.org/10.6840/cycu200901172

摘要

大黃素是存在於蓼科植物掌葉大黃之根莖部位中蔥醌類的一種主要成分，大黃素已經被發現許多效用，例如有抗癌、抗發炎、促進利尿和血管舒張的功效。在本研究中以多種實驗探討大黃素對於小鼠囊胚的毒性及後續胚胎發育之影響。結果發現，小鼠囊胚與50和75μM大黃素共培養後平均細胞數明顯的減少且凋亡細胞顯著的增加，同時實驗結果也顯示了大黃素有誘發氧化壓力上升並造成粒線體膜電位喪失的作用，下游的caspase-9和caspase-3也因而被活化。結果顯示，我們可以得知大黃素初期是透過氧化壓力增加及之後與粒線體依賴有關的凋亡途徑對小鼠囊胚體外發育造成傷害。

關鍵字

凋亡 ； 大黃素 ； 囊胚

並列摘要

Emodin is one of the main active components (anthraquinones) contained in the root rhizome of Rheum palmatum L. Emodin exhibits a wide range of pharmacological effects, including anti-cancer, anti- inflammatory, diuretic and vasorelaxant. In this study, we examine the cytotoxic effects of emodin in mouse blastocysts and subsequent early development. Blastocysts treated with 50 or 75 μM emodin showed significant increases in apoptosis and significant decreases in total cell number. Experiments in blastocysts show that emodin induces apoptosis via ROS generation, loss of mitochondrial membrane potential, and activation of caspase-9 and caspase-3. These findings show for the first time that emodin induces ROS generation and mitochondria-dependent apoptotic processes and causes developmental injury in mouse blastocysts in vitro.

並列關鍵字

apoptosis ； emodin ； blastocyst

參考文獻

Arathoon, E.G., Mejia, C.R., RM, Y.M., Hernandez, J.E., Turner, G.J., and Kitchen, L.W. (1994). Serum HIV-1 p24 levels and body weight measurements before and after 4 weeks of diethylcarbamazine treatment given to HIV-1 seropositive persons. Int J Antimicrob Agents 3, 275-278.

Aschkenazi, S., Straszewski, S., Verwer, K.M., Foellmer, H., Rutherford, T., and Mor, G. (2002). Differential regulation and function of the Fas/Fas ligand system in human trophoblast cells. Biol Reprod 66, 1853-1861.

Ashton, S.V., Whitley, G.S., Dash, P.R., Wareing, M., Crocker, I.P., Baker, P.N., and Cartwright, J.E. (2005). Uterine spiral artery remodeling involves endothelial apoptosis induced by extravillous trophoblasts through Fas/FasL interactions. Arterioscler Thromb Vasc Biol 25, 102-108.

Beck, F., Erler, T., Russell, A., and James, R. (1995). Expression of Cdx-2 in the mouse embryo and placenta: possible role in patterning of the extra-embryonic membranes. Dev Dyn 204, 219-227.

Brune, B. (2003). Nitric oxide: NO apoptosis or turning it ON? Cell Death Differ 10, 864-869.