- 學位論文
探討配體置換、老化以及溫度效應對於螢光奈米金團簇螢光特性之影響
Study on the Influence of Ligand Exchanging, Aging and Temperature Effects of the Fluorescence Property of Fluorescence Gold Nanocluster.
摘要
由於半導體量子點優異之光學特性，已有許多研究於生物標定等方面，但也有研究指出，因為半導體量子點合成之材料可能造成細胞毒性，因此侷限了在生物醫學等領域之發展。因此接下來較多議題為發展不具毒性之螢光奈米材料。近年來研究發現，當金進入奈米等級之後，在其表面接上具有硫醇基分子或樹枝狀高分子，便會使其具有螢光特性，且藉由改變硫醇基數目、分子結構或是末端官能基後，將會改變其螢光特性，此些研究將螢光奈米金團簇之發光機制指向＂Ligand-Metal Charge Transfer＂（LMCT）。 因此在本研究中，首先針對現有之螢光奈米金團簇（Au@DHLA），挑選具有硫醇基之碳鏈分子（Tiopronin / 12-MDA），在有機相以及水相環境中利用配體置換反應，使其與奈米金團簇表面產生反應，進而改變其螢光特性，提升其螢光強度；接著討論在配體置換反應時，環境溫度對於其螢光特性之影響；以及探討老化作用（aging）對於配體置換前後之螢光奈米金團簇，螢光特性為何會有增強之現象。
並列摘要
Semiconductor quantum dots are widely used for cell labeling due to their unique photophysical properties. However, cytotoxic of quantum dots limited its bioapplication because of the toxic precursor during synthesis. Therefore, more researchers have focused on developing new nanomaterials with low toxicity problems and fluorescent properties. Recently, some researches indicate that gold has fluorescent properties when its size become nanoscale with binding alkanethiolate or dendrimer on the surface of gold nanoclusters. Those researches indicate the fluorescence mechanism of gold nanoclusters like “Ligand Metal Charge Transfer＂（LMCT）. In this study, we select the molecule with alkanethiolate to proceed the ligand exchange reaction in organic and aqueous solvent for Au@DHLA. By the interaction with gold and alknethiolate to change the fluorescence properties and enhance fluorescence intensity. Further, we research the fluorescence’s variation of ligand exchange with control temperature, and the fluorescence’s variation by aging effect.