金屬有機凝膠作為酵素微反應器的開發與應用

Development and Application of Metal-Organic Gel on Immobilized Enzyme Microreactor

林育萱(Yu-Hsuan Lin)

指導教授 ： 林嘉和 ； 劉婉舲

中原大學/理學院/化學研究所/碩士(2018年)

https://doi.org/10.6840/cycu201800816

摘要

金屬有機凝膠(Metal-Organic Gel, MOGs) 為金屬離子或金屬團簇與有機配位基形成奈米級金屬有機顆粒或微孔奈米金屬有機骨架的凝膠材料。近幾年逐漸應用在氣體儲存、染料吸附、催化等方面，目前為止，尚未有MOGs作為酵素微反應器基材的研究發表，故本研究將MOGs開發為酵素微反應器，並將其應用至澱粉水解反應。
研究第一部分進行MOGs合成條件探討及鑑定，並利用物理吸附法固定α-澱粉酶，形成酵素微反應器。第二部份利用MOGs形成的酵素微反應器進行澱粉水解反應，並進行優化反應條件。實驗結果得知，AlBDC- 8及AlBDC- 9所製備的酵素微反應器具有最佳澱粉水解效能，產率可達到98 % 。隨後，研究第三部分進行微反應器之裝置化開發，以簡化微反應器重複使用之流程，裝置化後的α-澱粉酶在第一次的催化反應可達到98 %的水解效能，重複使用十次後，相對產率仍有76 %。
本實驗首次將MOGs開發為酵素微反應器，並成功應用至澱粉水解反應，其具有高產率、高穩定性、製備快速及分離產物簡易等優勢，裝置化後的酵素微反應器也具備商業化及工業化之潛力。

關鍵字

澱粉酶 ； 澱粉 ； 金屬有機凝膠

並列摘要

Metal-organic gels are constructed from metal ions or metal clusters and organic ligands to form permanent hierarchically porous structures such as micropores and mesopores through various non-covalent interactions. In recent years, the application of MOGs has been limited for gas storage, dye adsorption, catalysis, and chromatography. Since MOGs application for enzyme immobilization has not been reported, this study focused on the development of MOGs as an enzyme microreactor for starch hydrolysis. In the first part of this study, optimization on the synthesis and characterization of MOGs was carried out followed by enzyme immobilization via physical adsorption to form an enzyme microreactor. The second part of this study involves the optimization of the fabricated microreactor for starch hydrolysis. Based on the results, the enzyme microreactor prepared from aluminum based MOGs (AlBDC-8 and AlBDC-9) demonstrated the best performance for starch hydrolysis, which reaches up to 98%. Subsequently, a microreactor system was developed to simplify the reuse process of microreactors. Interestingly, the enzyme miroreactor system can be reused for at least 10 times with relative yield of 76%. This study demonstrated. The application of MOGs as enzyme support for the first time and applied in starch hydrolysis with high yield, high stability, ease of preparation and separation of products, suggesting its high potential for industrial application.

並列關鍵字

amlase ； starch ； MOG

參考文獻

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