具無線傳輸之光譜讀出系統應用於生物感測之設計實現

Design and Implement Wireless Spectral Readout System for Bio-sensing

吳冠緯(Guan-Wei Wu)

指導教授 ： 鍾文耀

中原大學/電機資訊學院/電子工程研究所/碩士(2018年)

https://doi.org/10.6840/cycu201800716

摘要

本研究設計及實現光譜讀出系統應用於生物測量及分析，使用局域表面電漿共振 (Localized Surface Plasmon Resonance) 透過其奈米結構之特性，可用於生物感測並將數據分析並顯示於設計之使用者介面，實現遠端光譜感測之功能。本研究可分為兩大架構：前端可攜式光譜讀出生物感測裝置，以及後端透過架設資料庫來對資料進行分析及儲存之數據分析處理系統。前者主要感測技術為局部表面電漿共振(LSPR)感測晶片，其表面是透過奈米金粒子所構成，利用易於附著生物分子之特性，使我們能夠透過其光譜之偏移量來分析待測樣本之數量。透過樣本流體注射平台，將待測樣本注入待測區域內與LSPR平台進行反應，再透過微光譜儀量測其結果。透過軟體開發將所量測之訊號無線傳輸上傳至資料庫。於資料庫端對量測之數據進行校正及分析後將光譜結果轉換成光譜圖、吸收度圖以及偏移圖，再透過網頁做最後的資料呈現，達到遠端進行即時數據檢測分析之功能。本研究同時具備易於攜帶、易於操作、即時讀出等等優勢，也能實現長距離的即時讀出檢測。不論是對於疾病感測還是對於細菌繁殖量或是殺菌藥物測試都能夠有重大之貢獻。

關鍵字

局域表面電漿共振、生物感測、無線傳輸 ； 光譜讀出 ； 可攜式 ； 吸收度 ； 資料庫 ； 網頁

並列摘要

The design and implementation of a wireless readout circuit system for bio-sensing, using Localized Surface Plasmon Resonance (LSPR), analysis and visualization of data using graphical user interface, website for mobile phone along with a database for data storage is presented in this project. In this study, the system can divided into two architectures: front end portable light spectrum readout bio-sensing system and the data processing system for analysis, visualization and storage of the results by a database. The LSPR platform is based on the gold nanoparticles, which have special feature to allow the bio-molecules such as proteins and DNA to attach on the surface of chip. In this research, the detection of the bio-molecules from the sample can be achieved by observing the shifts in the LSPR spectrum, allowing the sample to react with the LSPR chip through microfluidic channel, and subsequently measuring the spectrum by a micro-spectrometer. The data is updated remotely in real time and the designed website, app and graphical user interface allows easy visualization and analysis of the data. The main contribution of the system designed provides convenient, portable and easy to operate not only for disease detection but also a useful for the detection of pathogens and drug discovery.

並列關鍵字

LSPR, Biosensing,Portable ； Spectral readout ； Wireless ； Absorbance ； Database ； Website

參考文獻

[1] W. H. Organization, "Ebola response roadmap situation report update 4 November 2015," ed: WHO, 2015.

[2] Ebola (Ebola Virus Disease). Available: https://www.cdc.gov/vhf/ebola/history/chronology.html

[3] How Do Doctors Test for Ebola? Available: https://www.livescience.com/48141-how-doctors-test-for-ebola.html

[4] M. A. Almasi, S. M. Hosseyni-Dehabadi, and M. Aghapour-ojaghkandi, "Comparison and evaluation of three diagnostic methods for detection of beet curly top virus in sugar beet using different visualizing systems," Applied biochemistry and biotechnology, vol. 173, pp. 1836-1848, 2014.

[5] R. D. S. Luciene C Scherer, Antonio Ruffino-Netto, Maria LR Rossetti, Claudia Vater, Paul Klatser and Afrânio L Kritski, "Cost-effectiveness analysis of PCR for the rapid diagnosis of pulmonary tuberculosis," BMC Infectious Diseases, 2009.