大氣電漿於鈦金屬材料表面改質及促進骨細胞貼附之研究

To study the surface modification of titanium on the improvement of osteoblast adhesion using non-thermal atmospheric plasma

陳姿任(Zi-Ren Chen)

指導教授 ： 王明誠

中原大學/工學院/生物醫學工程研究所/碩士(2019年)

https://doi.org/10.6840/cycu201900145

摘要

隨著科技的進步與發展，近年來植牙已是目前最多缺牙病患選擇的治療方式，但植牙還是有將近一成的失敗率，根據植牙手術失敗的原因中了解到若能縮短骨整合的時間，將會有效提高植牙手術的成功率。根據文獻，大氣電漿能活化材料表面的親水官能基，因此可增加其表面親水性，亦不會對材料本身特性造成破壞。本研究以新型開發之大氣電漿設備處理鈦金屬表面，目的在於改善牙科植體親水性，進而提升其骨整合能力，藉由電漿設備的參數分析，發現在功率1000 W、氣流量0.2 MPa及處理距離為10 mm時為最佳處理參數；以熱電偶溫度計測量在電漿處理90秒時材料表面溫度為90.03 ℃，此溫度不會對鈦金屬造成熱相變。利用光學放射光譜儀(Optical Emission Spectroscopy, OES)分析電漿激發時所產生的電漿物種(Plasma species)，而物種中的活性氧物種(Reactive Oxygen Species, ROS)是提升材料表面親水的主要原因，結果檢測到包括在波長297.62 nm與309.00 nm的OH基及777.32 nm、845.75 nm與927.28 nm的單態氧等；藉由靜態接觸角分析，發現於短時間(10s)電漿處理後的材料表面接觸角度就可以從原本的67°下降至10°左右近乎超親水的效果，並進行親水性退化性的分析，由結果得知在電漿處理後24小時，其材料表面接觸角度會退化至50°左右的穩定角度。材料表面分析利用AFM檢測結果顯示電漿處理組與未處理組的Ra值皆為14 nm，並透過ESCA分析得知電漿處理組相較於未處理組的O/C比上升、N/C比沒有差異。藉由MTT評估細胞活性發現電漿組的結果皆比控制組好，且依據統計分析結果發現處理後培養24小時的結果與控制組有顯著上的差異。最後本研究成功使用此電漿設備有效處理鈦金屬表面，以達到對細胞貼附生長有利的條件。

關鍵字

大氣電漿 ； 鈦金屬 ； 骨母細胞

並列摘要

Owing to new technological advances, dental implantation has gained popularity to restore edentulous area. However, the failure rate of implantation is nearly to 10 %. According to the reason of dental implantation failure, it is found that shortening the osteointegration time may help to improve the success rate of dental implantation efficiently. From literature review, the hydrophilic functional group of the material surface can be activated by the atmospheric plasma and still not to damage the properties of the material. Therefore, the purpose of this study is to use novel developed atmospheric plasma equipment for the treatment on the surface of the Titanium, which can help to improve the hydrophilic nature of the dental implant and osteointegration capability. From the plasma equipment analysis, it is found that the best treatment parameters are at 1000 W power consumption, 0.2 MPa air pressure and 10mm treatment distance. The temperature of the surface material measured by thermocouple thermometer is 90.03 °C under 90 s of treatment period and does not cause any phase change under such a high temperature. The OES is used to analyze the production of the plasma species from the excitation of the plasma, particularly ROS is the main reason to elevate hydrophilic properties on the material surface. The test results showed that OH- species are existed at 297.62nm, 309.00nm wavelength, and O- at 777.32 nm, 845.75 nm, 927.28 nm wavelength. The contact angle of the material surface is reduced from 67° to roughly 10° at a short period of treatment time(10 s) and decay around to 50° after 24 hours. The analysis of the material surface using AFM showed that the Ra value of the plasma group and the controlled group is 14 nm, ESCA analysis showed the plasma group has increase the O/C ratio and no differences in N/C ratio. Assessing the cell viability from the MTT showed that plasma group has better cell viability compared to the control group significantly after 24 hours of cell cultivation. At last, the research has successfully used the plasma equipment to process the surface of the Titanium for a better cell adhesion and growth in a favorable condition.

並列關鍵字

atmospheric plasma ； Titanium ； Osteoblast

參考文獻

1.馮聖偉, 生醫材料（六）：牙科植體發展新境界. 2014.

2.李凌珺, 別讓牙齒打敗你. 2017: p. 11.

3.家庭醫生在線, 缺牙是怎麼導致的 當心缺牙的種種危害. 2016.

4.柯心卉, 全球牙科醫材市場穩定成長，2016～2021年複合成長率為5.6%. 2017.

5.吳宗信, 電漿-現代與未來科技發展的隱形發動機. April 14, 2013.