This work focus on the pattern transferring of sol-gel indium oxide by nanoimprint technology. The nanowire and honeycomb structures and the double-gate ion sensitive field-effect transistors were fabricated. With the capacitive coupling effect and the nanowire/honeycomb structures high-sensitive DG ISFETs can be achieved. The results showed that the nanowires and honeycomb structures with the line width of ~158 and ~89nm were fabricated successfully. The sensitivity and AF of DG ISFETs were about −218 mV⁄pH and 2.7~13.6. This technique will be suitable for the future biochemical sensors and the wearable elements applications.