聚合離子液體與苯二甲藍鈷修飾網版印刷電極催化硫醇化合物之研究

Electrocatalytic oxidation of 2-mercaptoethanol by poly ionic liquid and cobalt phthalocyanine modified screen-printed carbon electrodes

王裕榮(Yu-Jung Wang)

指導教授 ： 鄭淑華

國立暨南國際大學/科技學院/應用化學系/碩士(2013年)

https://doi.org/10.6837/NCNU.2013.00187

摘要

本研究結合CoPc (cobalt phthalocyanine)與聚合離子液體 (poly ionic liquid, PIL)來修飾網版印刷電極(screen print carbon electrode, SPCE)，首先在SPCE電極表面熱聚合兩種離子液體單體成為聚合離子液體成為SPCE/PIL電極，爾後在電極表面塗佈CoPc修飾溶液，烘烤至乾燥即可得到SPCE/PIL/CoPc修飾電極，再利用循環伏安法前處理後提高電極對硫醇分子的活性。此修飾電極可在pH7觀察到一對來自於CoPc中心鈷金屬(cobalt)的CoII/CoI氧化還原對，經由CoPc作為媒介物使此電極對硫醇分子具有電催化活性，可大幅降低其氧化電位與增加氧化電流，利用循環伏安法(cyclic voltammetry, CV) 在pH7至pH12的緩衝溶液中分別偵測穀胱甘肽(Glutathione, GSH), 2-巰基乙醇(2-Mercaptoethanol, 2-ME), 半胱氨酸(Cysteine)等三種硫醇分子，進一步使用FIA (flow injection analysis)在pH 7緩衝溶液中以電位0.1 V分析2-ME，可得到工作範圍為5 ?M 至1000 ?M，靈敏度(sensitivity) 0.01 ?A/?M與偵測極限為0.69 ?M。

關鍵字

離子液體 ； 聚合離子液體 ； 苯二甲藍鈷 ； 2-巰基乙醇 ； 穀胱甘肽半胱氨酸 ； 循環伏安法 ； 流動注射分析法

並列摘要

In this study, we prepared the poly ionic liquid (PIL) and cobalt phthalocyanine (CoPc) modified electrodes, showing characteristics of electrocatalytic oxidation of thiol compounds in aqueous solution. The bi-layer modified electrode (SPCE/PIL/CoPc*) was constructed by polymerization the ionic liquid monomers on the screen-pirnted carbon electrode at 80℃ for 5 hours, casting the CoPc solution on the electrode surface, dried in the oven at 50℃ for 3hrs, then electrochemical pretreatment by potential scanning. The poly ionic liquid layer behaves as a bridge to improve the electode conductivity between SPCE and CoPc. The SPCE/PIL/CoPc* modified electrode showed excellent catalytic activity for the thiols (cysteine, GSH, 2-ME) compounds in pH 7.0 to pH 12.0 buffer solution. The electrochemical behavior of the modified electrode was invstigated using cyclic voltammetry and flow injection analysis methods. By the FIA method, the detection limit is 0.69??M for 2-ME.

並列關鍵字

ionic liquid ； poly ionic liquid ； cobalt phthalocyanine ； 2-mercaptoethanol ； glutathione ； cysteine ； cyclic voltammetry ； flow injection analysis

參考文獻

[1] Lane, R. F.; Hubbard, A. T., The Journal of Physical Chemistry 1973, 77, 1401-1410.

[2] Murray, R. W., Accounts of Chemical Research 1980, 13, 135-141.

[3] Opallo, M.; Lesniewski, A., Journal of Electroanalytical Chemistry 2011, 656, 2-16.

[4] Green, O.; Grubjesic, S.; Lee, S.; Firestone, M. A., Polymer Reviews 2009, 49, 339-360.

[5] Pandurangachar, M.; Kumara Swamy, B. E.; Chandrashekar, B. N.; Gilbert, O.; Sherigara, B. S., Journal of Molecular Liquids 2011, 158, 13-17.