鈷紫質修飾網版印刷碳電極電催化2-硫氫乙醇氧化
反應之研究

本篇論文製備鈷紫質網版印刷電極，並應用於在水溶液條件下偵測二硫氫乙醇(2-ME)，裸電極先在中性溶液下進行活化，再將鈷紫質和全氟磺酸混合溶液滴在活化電極表面上，我們用循環伏安法探討修飾電極偵測2-ME的電化學行為，並發現鈷紫質網版印刷電極對於2-ME有很好的催化效果。進一步利用FIA系統在鹼性溶液中偵測2-ME，發現修飾電極具有良好的偵測極限0.2 µM、靈敏度0.0093 µAµM-1以及較廣的偵測範圍1~1000 µM，此外修飾電極也不會受到尿酸、咖啡因、穀胱甘肽的干擾。

關鍵字

2-硫氫乙醇；網版印刷電極；鈷紫質；全氟磺酸

並列摘要

We report here the electrochemical determination of 2-mercaptoethanol (2-ME) in alkaline aqueous solution by using cobalt(III) porphyrin/Nafion screen printed carbon electrodes (SPCE). Bare SPCE were electro-activated by potential scans ranging from -0.6 to 1.0 V for 10 cycles in pH 7 buffer solution. Then, a Nafion solution containing cobalt porphyrin was dropped onto the pre-actived electrode surfaces. Cyclic voltammetrograms of 5.0 mM 2-ME was studied in various buffer solutions at the modified electrodes. It was observed that the modified electrodes showed good electrocatalytic activity toward the oxidation of 2-ME. Flow injection amperometry (FIA) was studied in pH 12 buffer solution at the optimized conditions. Good analytical features were obtained, including a wide linear range (1~1000 µM), a low detection limit(0.2 µM) and high sensitivity (0.0093 µAµM-1). In addition, the interferent species, such as uric acid, glutathione and caffeine showed insignificant effect on the detection signals.