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  • 學位論文

利用電場分離不同大小顆粒的初步探討

Preliminary Study of Electric Field to Separate Particles of Different Sizes

指導教授 : 傅傳博

摘要


利用微流體電泳的觀念,可藉由電壓切換來控制分析物在通道中的移動方向,進而達到分離流程。跟據實驗結果,控制時間與電壓得到最適化進樣電壓205 V,其進樣時間依顆粒大小有所不同,分別為SiO2顆粒大小為1、2、4 µm,分別進樣時間為50、80、110 秒 (S),顆粒為36.7、30.7、20.7 顆。以最適化進樣探討最適化的分離時間電壓得到以350V 分離最好,依照顆粒大小分別為1、2、4 µm,3、4、5 分鐘 (min),分離10.3、8.0、6.3 顆粒數。

並列摘要


We have synthesized SiO2 particles by Sol-Gel method. By the variation of TEOS and NH4OH concentration we obtained 1,2 and 4 µm size SiO2 particles. During this experiment, microchip electrophoresis can be controlled by the switching the voltage toward the direction of the analyte using buffer K2HPO4 / KH2PO4 ( pH 8.0,0.1 M ) in the channel. According to the result of experiment, by means of time control and voltage is 205V,the sampling time depends on different size of SiO2 particles, which are 1,2 and 4 µm, the sampling are respectively 50、80、110 seconds, the number of particles are 36.7、30.7、20.7. With the method of the most suitable sampling, we discover the best voltage of separation time is 350V, In accordance with the dimension of particles are 1,2 and 4 µm, 3、4、5 mins and to separate 10.3、8.0、6.3 particles.

並列關鍵字

Sol-Gel method SiO2 particles Electric Field

參考文獻


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