- 學位論文
毛細管微胞電動層析法-比較陰離子及陽離子界面活性劑對類固醇分析的影響之研究
Comparison of the use of anionic and cationic surfactants for the separation of steroids based on MEKC and sweeping-MEKC modes
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摘要
為了增進類固醇分離的選擇性與靈敏度，並且討論分析物在電泳時產生不同的遷移速率，本實驗比較使用不同陰離子及陽離子界面活性劑在毛細管微胞電動層析法中對類固醇分離的影響。利用毛細管電泳/紫外光吸收偵測法分離且偵測到六個混合的類固醇-progesterone、17-hydroxy progesterone、11-deoxycortisol、corticosterone、cortisol、cortisone。類固醇的電泳遷移速率受到背景溶液的酸鹼值、界面活性劑的種類以及分析電壓為正電或負電所影響，這些因素會影響到EOF的大小及方向，或使微胞的遷移速率以及遷移方向發生改變，本實驗將對影響類固醇在電泳時遷移速率的主要因素作討論及整理。運用毛細管電泳掃集技術已大為降低對類固醇的偵測極限，且成功在老鼠及人體血液及尿液樣品中偵測到類固醇的含量。
並列摘要
In attempts to improve the selectivity and sensitivity of steroid separation and to determine their migration order, a comparison of the use of anionic and cationic surfactants based on the MEKC and sweeping-MEKC modes was made. A mixture of six steroids (progesterone, 17-hydroxy progesterone, 11-deoxycortisol, corticosterone,cortisone, and cortisol) could be separated and detected by means of the CE/UV-absorption method. The order of migration time for these steroids was compared under various conditions, including acidic/alkaline buffers, anionic/cationic surfactants, and positive/negative applied voltage, causing the direction of the EOF and the migration of micelles to change. The major rules for generally predicting the migration order of steroids are summarized. The detection limits were significantly improved when the sweeping-MEKC mode was applied. The six steroids were also determined in samples of female urine and blood. The extraction procedures used for the real samples and the CE conditions for their separation were all optimized and the data are reported herein.
參考文獻
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延伸閱讀
- 陳詩茹（2004）。毛細管電泳暨間接螢光離子分析法與金奈米粒子硫醇感測法〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2004.00059
- 周育緯（2009）。Ⅰ. 毛細管電泳法對藥物於生物檢品中及光學異構物之分析研究Ⅱ. 高效能液相層析法對抗腫瘤藥物Mitomycin C與Epirubicin之同時分析研究〔博士論文，高雄醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0011-2007200914313300
- 林楓閔（2004）。毛細管氣相層析及毛細管電泳用衍生試劑對trans,trans-muconic acid、碘化物及胺基藥物之分析應用〔博士論文，高雄醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0011-2903200614002426
- Soylak, M., Colak, H., Tuzen, M., Turkoglu, O., & Elci, L. (2006). Comparison of Digestion Procedures on Commercial Powdered Soup Samples for the Determination of Trace Metal Contents by Atomic Absorption Spectrometry. 藥物食品分析, 14(1), 62-67. https://www.airitilibrary.com/Article/Detail?DocID=10219498-200603-14-1-62-67-a
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