沒食子酸(gallic acid)對高果糖飼料誘導高血糖大鼠血糖及脂質代謝之影響

Effect of gallic acid on hyperglycemia and lipid metabolism in high fructose diet-induced diabetic rats

楊恆叡

指導教授 ： 沈賜川

國立臺灣師範大學/教育學院/人類發展與家庭學系/碩士(2014年)

摘要

高血糖症(hyperglycemia)為糖尿病(diabetes mellitus )主要病徵，常伴隨胰島素阻抗(insulin resistance)，使肝臟、肌肉、脂肪等週邊組織對於胰島素敏感性(insulin sensitivity)降低，影響血糖利用，導致人體長期對於葡萄糖耐受性不良。沒食子酸(gallic acid)為苯甲酸(benzoic acid)的衍生物，化學名3,4,5-trihydroxybenzoic acid，廣泛存在於各類植物中。近期研究發現酚酸類化合物具有抗氧化及改善葡萄糖攝入(glucose uptake)之功效。本研究探討沒食子酸對高果糖飼料誘導高血糖大鼠降血糖及脂質代謝之影響。餵食66％高果糖飼料12週後之Wistar大鼠分為四組(每組6隻)，分別為：高果糖組、臨床糖尿病用藥(pioglitazone hydrochloride)組、低劑量(10 mg/kg B.W.)沒食子酸組、高劑量(30 mg/kg B.W.)沒食子酸組，糖尿病用藥與沒食子酸每日管餵一次，並與控制組(正常大鼠)作比較。老鼠於實驗進行17週後犧牲，結果顯示，糖尿病大鼠餵食30 mg/kg B.W.沒食子酸4週後，相較於高果糖組有明顯降低禁食血糖(fasting glucose) (從224.17 mg/dL降至84.83 mg/dL)及降低高胰島素血症 (從0.99 μg/L降低至0.42 μg/L)的效果；體脂肪方面，採集腎周脂肪(perirenal fat)及副睪脂肪(epididymal fat)進行分析，高劑量gallic acid組相較於高果糖組，明顯降低腎周脂肪(從6.7 mg降低至5.58 mg) 及副睪脂肪(從2.95 mg降低至2.02 mg)；於脂肪組織中胰島素訊息傳遞方面，以西方墨點法偵測蛋白質表現量，結果顯示，餵食高劑量沒食子酸組相較於高果糖組可提昇54％葡萄糖轉運蛋白(glucosetransporter-4, GLUT4)表現量。综合以上結果推論，沒食子酸對於高果糖誘導高血糖大鼠具有降低血糖及調控脂質代謝之效果。

關鍵字

沒食子酸 ； 糖尿病 ； 胰島素訊息傳遞 ； 胰島素阻抗 ； 高血糖

並列摘要

Hyperglycemia is a major symptom of diabetes mellitus. It often associated with insulin resistance which will lower the insulin sensitivity of liver, muscles and adipose, futher effect the assimilation of blood sugar and lead to long-term impaired glucose tolerance (IGT). Gallic acid is the derivatives of benzoic acid (formula: 3,4,5 -trihydroxybenzoic acid) exists in various kinds of plants. Recent researches demonstrated that phenoic compounds exhibit anti-oxidation and improve glucose uptake activities in vitro. This study investigated the effect of gallic acid on hypoglycemia and lipid metabolism in high-fructose diet (HFD)-induced diabetic rats. After fed the 66% fructose for twelve weeks, the Wistar rats were devided into 4 groups (6 rats each group): HFD, pioglitazone hydrochloride, low dose (10 mg/kg B.W.) gallic acid, high dose (30 mg/kg B.W.) gallic acid groups. Rats fed the pioglitazone hydrochloride and the high dose (30 mg/kg B.W.) gallic acid once a day and were sacrified after experimenting for 17 weeks. The the fasting glucose and insulin levels of HFD rats significantly reduced (the former declined from 224.17 mg/dL to 84.83mg/dL, the latter declined from 0.99 μg/L to 0.42 μg/L) after a 4-week feeding of gallic acid (30 mg/kg B.W.). The perirenal and epididymal fats of the high dose gallic acid group significantly decreased compared to the HFD rats (the former declined from from 6.7 mg to 5.58 mg, the latter declined from 2.95 mg to 2.02 mg). The western blot analysis was conducted to evaluate the effect of gallic acid on insulin signal transduction pathway in adipose tissue of HFD rats. The high dose gallic acid increased 54％ glucose transporter-4 (GLUT4) compared with the HFD rats. We postulate that gallic acid may alleviate hyperglycemia and modulate lipid metabolism of adipose in HFD rats.

並列關鍵字

diabetic rats ； gallic acid ； hypoglycemia ； insulin signaling ； lipid metabolism

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