The purpose of this study was to investigate the effect of different ratios of eccentric contraction training on upper arm’s muscle performance. Twenty eight male volunteers(mean age were 21.07±1.86 years)were recruited to participate in this study and randomly assigned into three groups:group A(ratios of concentric/eccentric was 1:1;two seconds:two seconds );group B(1:2;two seconds:four seconds);group C(1:3;two seconds:six seconds). Before and after training, subjects accepted pre-test and post-test using Biodex isokinetic force machine to measure concentric and eccentric contraction peak torque(PT )of biceps and triceps. The measurements were set at two different speed of 60°•S-1 and 120°•S-1. The 8-week training regimens were two days a week, three sets every day, 10 repetitions every set at intensity of 70% of 1RM with dumbbell. The upper-arm circumference(CIR)and skinfold thickness of biceps and triceps were measured too. The dependent factors were two different testing speed and three different ratios of eccentric contraction. The data were analyzed using a two-way [ speed(60°•S-1 and 120°•S-1)×group(A,B and C)] mixed factorial ANOVA. Significant difference of PT was found between group B and A, group B and C in 60°•S-1 biceps eccentric contraction. Group B was superior to group A and C. But biceps concentric, triceps concentric, and triceps eccentric contraction were no significant difference of PT in 60°•S-1. There was no significant difference of PT existed among A , B and C groups in 120°•S-1 .No significant difference was found of CIR and skinfold thickness of biceps and triceps among different groups. The study suggests that resistance trainers should use 1:2 ratio of eccentric contraction to make the best eccentric contraction of PT when training their upper arm.