多頻道碳六十固定化酵素石英壓電生化感測器之研製與應用

Preparation and Application of Multi-channel Piezoelectric Crystal Bio-sensor Based on Immobilized Fullerene C60-Enzyme for Bio-Species

洪銘聰(Ming-Tsung Hung)

指導教授 ： 施正雄

國立臺灣師範大學/理學院/化學系/碩士(2005年)

摘要

無資料

關鍵字

石英壓電感測器 ； 碳六十 ； 固定化酵素 ； 葡萄糖氧化酵素 ； 尿素分解酵素 ； 過氧化氫分解酵素

並列摘要

Glucose, urea and hydrogen peroxide are important bio-species in the human body. In this study, a multi-channel piezoelectric crystal bio-sensor with the home-made computer interface for data processing was prepared and employed to detect separately various bio-species, e.g. glucose, urea and H2O2. The multi-channel PZ system includes two working electrodes and two reference electrodes and one oxygen electrode. Various immobilized fullerene C60-enzymes, e.g. C60-glucose oxidase, C60-Urease and C60-catalase, were synthesized and applied to catalyze oxidation of glucose, decomposition of urea and H2O2, respectively in this piezoelectric bio-sensor system. The C60 and C60-Cryptand-22 coated piezoelectric crystals were used to detect various products of these enzyme-catalyzed reactions. The oscillated frequency decreased due to adsorption of product catalyzed by immobilized C60-enzyme on the coating material. The C60-enzyme coated PZ bio-sensor exhibited quiet good selectivity for these bio-species. Other interfering compounds e.q. cysteine, tyrosine, uric acid and galactose in sample solutions showed nearly no interference with these C60-enzyme coated PZ bio-sensor. The activity of immobilized C60-enzyme can be hold over 90% in 15 days. The home-made multi-channels Piezoelectric crystal bio-sensor showed good detection limit of 12.37μg/mL and R2 values of calibration curve were more than 0.978. The piezoelectric crystal bio-sensor system can distinguish individual components in the mixed samples. Furthermore, multivariate linear regression (MLR) was employed to make quantitative analysis for these biospecies.

並列關鍵字

QCM ； Fullerene ； Immobilized Enzyme ； Glucose Oxidase ； Urease ； Catalase