去氫表雄固酮對專一性抗體反應調節功能的探討

Study on the role of dehydroepiandrosterone in regulation of specific antibody response

吳國宏(WU KUO HUNG)

指導教授 ： 曾哲明 博士

國立臺灣師範大學/理學院/生物學系/碩士(2000年)

摘要

中文摘要
去氫表雄固酮(DHEA)為腎上腺皮質網狀層分泌的主要雄性激素之一，在血清中則大部份以硫化物(DHEAS)存在，參與哺乳類體內多種生理反應。近年來的文獻指出，DHEA具有調節免疫反應的功能，並發現DHEA可作為老年人或年老小白鼠注射疫苗時的佐劑。然而，DHEA是否可增強幼年期動物之專一性抗體反應仍有待研究，且注射DHEA後，相關細胞激素的變化亦不明確。因此本實驗目的為研究DHEA在幼年小白鼠接受大腸桿菌(E.coli O78:H11)、三合一疫苗(DPT)或百日咳毒素(PT)的免疫注射時，是否可增強其專一性抗體反應，並進一步探討其與細胞激素的關係。實驗結果顯示，DHEA對於大腸桿菌或百日咳毒素引起之專一性抗體反應均可增強抗體反應；然而抗三合一疫苗抗體實驗中，顯示DHEA對三合一疫苗不具佐劑效應。感染過E.coli或以PT致敏的老鼠施打DHEA後，DHEA對E.coli或PT激發脾臟淋巴球製造IFN-g、IL-2與IL-4、IL-10等細胞激素的作用皆有加乘效應，可見DHEA的免疫增強功能可能是透過對細胞激素的調節。本研究不但證實DHEA可促進專一性抗體反應，且發現此免疫調節功能與細胞激素有關。

關鍵字

去氫表雄固酮

並列摘要

Abstract
Dehydroepiandrosterone (DHEA) is one of the major androgens secreted by adrenal cortex and play a multifunctional role in regulating physiological system in mammals. DHEA is predominantly converted to DHEA-sulfate (DHEAS) in serum . Recent reports demonstrated that DHEA is a potent immunomodulator. In addition, recent data showed that DHEA might act as an effective vaccine adjuvant in aged human and mice. However, the immunostimulatory effect on the children and young animal upon immunization remain to be studied. Furthermore, the profile of cytokine associated with DHEA-mediated regulatory effect remained to be defined. The major purpose of this study is to investigate the possible immunostimulatory effect of DHEA on the young mice immunized with Escherichia coli (E.coli O78:H11), combined diphtheria, tetanus, acellular pertussis vaccine (DPT vaccine) or pertussis toxin (PT), and to investigate the profile of cytokine associated with DHEA administration. Results indicated that DHEA has an augmented effect on the young mice immunized with Escherichia coli (E.coli O78:H11) or pertussis toxin (PT). However, DHEA did not show adjuvant effect on DPT vaccine immunized mice. In the subsequent study, the mice were infected with E. coli or challenged with PT, followed by subcutaneous injection with DHEA. Result indicated that DHEA synergistically enhanced E. coli or PT induced IFN-g, IL-2 and IL-4, IL-10 cytokine productions. Therefore, the immunoregulatory effect of DHEA might closely relate with its ability to modulate cytokine production. This study not only confirmed the ability of DHEA to regulate specific antibody response, but also demonstrated the role of DHEA in regulation of cytokine production.

並列關鍵字

dehydroepiandrosterone

參考文獻

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