The purpose of this study was to investigate how high school students perceived and responded to the concept of animal welfare in the zoo (taking Taipei Zoo as an example) after receiving the animal welfare teaching material designed in this study. Students' attitudes towards the use of animals and their understandings of animal welfare were assessed after their actual visit to the zoo site. Exploration was also on how the biology teacher interpreted and implemented the animal welfare teaching material. In this study, the questionnaire of pre-test and post-test of animal attitudes, the use of teaching materials, and the animal welfare evaluation work sheet for students’ actual visits to the zoo were used as the source of data collection and assessment tools. Analyses focused on whether high school students towards animal use can be improved after the implementation of this teaching material.