以石墨烯量子點調制表面電漿子能量於生物感測器之研究

Modulation of Surface Plasmon Resonance Energy Using Graphene Quantum Dots on Biosensors Application

吳瑋仁(Wu Wei-Ren)

指導教授 ： 邱南福

國立臺灣師範大學/理學院/光電科技研究所/碩士(2018年)

https://doi.org/10.6345/THE.NTNU.EPST.019.2018.E08

摘要

疾病的早期發現早期治療，是近代醫學的檢測與診斷的方向，生物醫學晶片能夠提供快速、準確、低成本的檢測，以實現精準醫療的目標。本研究提出利用石墨烯量子點(GQDs)結合表面電漿子共振(SPR)技術來開發能夠快速檢測胰臟癌疾病的生物晶片。
石墨烯量子點是二維的新穎材料，具有良好的生物相容性與優異的光致發光之螢光效益。因此本研究使用水熱合成法自行合成石墨烯量子點，並進行光學特性分析、元素分析以及形態分析驗的驗證，證實透過材料顆粒大小可以調控光致發光的螢光顏色。在石墨烯量子點修飾於金奈米粒子的實驗中，發現石墨烯量子點和金奈米粒子之間的交互作用，能誘導表面電漿子的能量轉移特性產生耦合現象，並藉由調制表面電漿子能量來控制螢光的色變，開發螢光生物感測技術。
石墨烯量子點藉由表面電漿子共振技術檢測生物分子間的交互作用，進行檢測胰臟癌腫瘤指標物CA19-9之免疫反應的實驗，透過快速低成本的檢測技術進而開發高靈敏度及高準確性之快速篩檢的生物感測器。本實驗最低檢測可達約10 Unit/ml的變化，且線性迴歸係數可達R2 = 0.9，透過此檢測技術即能快速診斷胰臟癌。由此實驗可證明石墨烯量子點具有良好的生物相容性，結合生物晶片亦能提高檢測的靈敏度，並能夠用於開發具有專一性的快速篩檢的癌症生物感測晶片。

關鍵字

石墨烯量子點 ； 金奈米粒子 ； 表面電漿子共振 ； 胰臟癌腫瘤指標物

並列摘要

Early detection of early detection of disease is the direction of detection and diagnosis of modern medicine. Biomedical chips provide fast, accurate, and low-cost detection for precise medical goals. This study proposes the use of graphene quantum dots (GQDs) combined with surface plasmon resonance (SPR) technology to develop biochips capable of rapidly detecting pancreatic cancer diseases.
Graphene quantum dots are two-dimensional novel materials with good biocompatibility and excellent photoluminescence fluorescence benefits. Therefore, this study used hydrothermal synthesis to synthesize graphene quantum dots and performed optical property analysis, elemental analysis and morphological analysis to verify that the particle size of the material can regulate the fluorescent color of photoluminescence. In the experiment of graphene quantum dots modified in gold nanoparticles, it was found that the interaction between graphene quantum dots and gold nanoparticles can induce the coupling phenomenon of the energy transfer characteristics of surface plasmons. Fluorescent biosensing technology was developed by modulating the surface plasmonic energy to control the color change of the fluorescent light.
Experiments were carried out to detect the immune response between pancreatic cancer tumor marker CA19-9 by using graphene quantum dots to detect the interaction between biomolecules by surface plasmon resonance technique. Develop high-sensitivity and high-accuracy biosensors for rapid screening through fast and low-cost detection technology. The minimum detection of this experiment can reach about 10 Unit/ml, and the linear regression coefficient can reach R2 = 0.9 . This detection technique can quickly diagnose pancreatic cancer. This experiment proves that the graphene quantum dots have good biocompatibility, and the biochip can also improve the sensitivity of detection, and can be used to develop a cancer screening biosensor chips with specific rapid screening.

並列關鍵字

Graphene quantum dots (GQDs) ； Gold nanoparticles (AuNPs) ； Surface plasmon resonance (SPR) ； Carbohydrate Antigen 19-9 (CA19-9)

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