This study is aiming at exploring the eyes movement process of college student when reading graphic layout of the automobile owner's manual. The researcher divided the graphic layout of the automobile owner's manual into four types: (a.) The pictures at right and the text at left; (b.) The pictures at left and text at right; (c.) The pictures at top and text at below; (d.) Text at top and the pictures at below. The researcher asked participants to read the automobile owner's manual which were mentioned. At the same time, the researcher operated eyes tracker to observe the process of the eyes movement to realize the reading time and the operating time. This study found out that the majority of the participants who chose horizontal layout (the ‘a’ and ‘b’ types) spent 43.8 seconds to read and 138.5 seconds to operate in average. In addition, the participants who chose vertical graphic layout (the ‘c’ and ‘d’ types) spent 27.7 seconds to read and 101.8 seconds to operate in average. In conclusion, the participants who chose the vertical layout would shorter than horizontal one.