N-乙醯半胱氨酸對斑馬魚仔魚側線毛細胞的影響

Effects of N-acetyl cysteine on Lateral Line Hair Cell of Zebrafish Larvae (Danio Rerio)

馮瑩茜(Phong Ying-Chiann)

指導教授 ： 林豊益

國立臺灣師範大學/理學院/生命科學系/碩士(2019年)

https://doi.org/10.6345/THE.NTNU.SLS.005.2019.D01

摘要

N-乙醯半胱氨酸（N-acetyl cysteine，NAC）是一種作為營養食品的抗氧化劑。在水產養殖上使用N-乙醯半胱氨酸餵食吳郭魚亦可以降低微囊藻毒素和柱孢藻毒素所產生的氧化壓力。此研究的目的是想要探討抗氧化劑NAC的潛在毒性及致死劑量，以便未來在魚類上作為抗氧化劑的應用。此外測試NAC對於新黴素所造成耳毒性傷害是否有保護作用。我們將斑馬魚長時間及短時間曝露於NAC，並觀察NAC對斑馬魚的影響。我們的研究結果顯示NAC會對斑馬魚仔魚造成死亡的現象，並且其藥效與濃度成正相關。另外，NAC對各個發育的指標都會帶來負面的影響，如存活率、孵化率、斑馬魚仔魚的體長及心跳速率。另外，使用FM1-43這活體染劑，可得知NAC在急性或是慢性處理下都會減少斑馬魚仔魚的毛細胞。急性NAC處理後會增加毛細胞的氧化壓力。除此之外，NAC亦會減少斑馬魚仔魚卵黃囊上的離子細胞密度。從以上的結果可得知不管是急性或是慢性的NAC處理都會對斑馬魚造成毒性傷害。

關鍵字

N-乙醯半胱氨酸 ； 抗氧化劑 ； 新黴素 ； 斑馬魚 ； 側線 ； 毛細胞 ； 耳毒性 ； 胚胎毒性

並列摘要

N-acetyl cysteine (NAC), an antioxidant used as a nutritional supplement, was used to protect tilapia from oxidative stress-induced by Microcystin and Cylindrospermopsin in the aquaculture sector. The aim of the present study was to investigate the potential toxicity and lethal dose of NAC before applying it to be an antioxidant in fish. Furthermore, we test for the protective effect of NAC against neomycin-induced ototoxicity. We investigate the embryonic toxicity of NAC on zebrafish larvae by acute or chronic exposure to NAC. Our results showed that NAC caused a lethal effect on zebrafish embryo in a dose-dependent manner. By the development assessments, survival rate, body length, and heart rate significantly decreased after the NAC treatment. By labeling hair cells with FM1-43, the hair cells number decreased in both acute and chronic NAC treatment groups. Oxidative stress of lateral line hair cell increased after acute NAC treatment. Moreover, NAC reduced the density of ionocyte on zebrafish yolk sac. Our findings demonstrate that NAC may cause toxicity to zebrafish in both acute and chronic treatment.

並列關鍵字

N-acetyl cysteine ； antioxidant ； neomycin ； zebrafish ； lateral line ； hair cell ； ototoxicity ； embryotoxicity

參考文獻

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