應用雷射散斑對比成像探討腫瘤微循環與雷射治療成效評估

Investigation of tumor microcirculation and evaluation of laser therapy with laser speckle contrast imaging

蔡勻富(Tsai Yun-Fu)

指導教授 ： 李亞儒 ； 蔡孟燦

國立臺灣師範大學/理學院/光電科技研究所/碩士(2019年)

https://doi.org/10.6345/NTNU201900540

摘要

腫瘤發展初期須藉由血管進行養分輸送，腫瘤細胞也會透過血管進行轉移，因此對於腫瘤早期發展異常新生血管為重要的診斷指標。雷射散斑對比成像(Laser speckle contrst imaging, LSCI)技術可以以非破壞性方式獲得生物組織血液動態微循環訊息，包含血管型態、流速、血氧飽和濃度等重要參數，因此在此研究中我們建立了一套雷射散斑對比顯微系統，利用雙波長雷射作為照明雷射並整合於商用顯微鏡可用於小動物活體量測，並透過所建立的演算法可以即時獲得上述血管微循環參數。另外，我們也將此套雷射散斑對比顯微系統應用於探討下列主題，包含外加壓力對於組織微循環的動態效應、腫瘤生長初期微循環的變化、即時監控與評估雷射於腫瘤治療可行性、連續波與脈衝式雷射治療特性比較等。由實驗結果已驗證組織微循環在外加壓力下所造成的動態變化可由此套系統觀測，腫瘤生長初期的微循環變化也可以透過成像結果可進一步量化流速變化、血管型態變化、血氧飽和濃度變化，經由雷射治療而觀察血管中血氧濃度變化，可以達到阻斷血液中的養分輸送以抑制腫瘤生長。相較於連續式雷射治療，脈衝式雷射治療可以達到漸進治療成效避免組織過度傷害。由此論文的結果可發現此套顯微成像系統可透過多項參數活體評估小動物疾病模型，也可以用於小動物治療模型，希望未來透過微小化工程預計將可用於人體即時量測。

關鍵字

腫瘤微環境 ； 雷射散斑對比成像 ； 血管成像 ； 雷射治療

並列摘要

The initial development of tumor needs blood vessels to transport nutrient. Tumor cells divert through blood vessels. Therefore, the newborn blood vessels are important index to diagnose in regard to the abnormal development of tumor. Laser speckle contrast imaging (LSCI) is the technology which can acquire the blood circle of organism organization through undamaged way. The information of blood circle includes blood vessels situation, flow speed, and blood oxygen saturated concentration. Thus, we form the LSCI system in this research. This system combines double wavelength laser to the microscope, which can be used on the tiny organism biopsy. Through the system and the calculation, we can get the blood circle of organism organization. In addition, we will use LSCI to the following topics, includes moment effect of organism organization with additional pressure, the organism organization variation in the initial development of tumor, the feasibility of immediately monitoring and evaluating tumor treatment, and the contrast of continuous wave and pulse laser treatments’ property. The result of this research confirms that organism organization variation by additional pressure can be observed by LSCI. The organism organization variation can further quantifies the variation of flow speed, blood vessels situation, and blood oxygen saturated concentration through imaging results. The observation of blood oxygen saturated concentration in the blood vessels by LSCI, which can obstruct the nutrient transportation in the blood vessels to keep down the development of tumors. In compare with the continuous laser treatment, pulse laser treatment can achieve the effect of gradual progress treatment to keep organization from being damaged. The result of this thesis indicates that LSCI can evaluate the disease model of tiny creatures through parameters. It also can be used on model treatment of tiny creatures. In the future, hope that infinitesimal project can be immediately used on human body treatment.

並列關鍵字

tumor microcirculation ； Laser speckle contrast imaging ； blood vessels imaging ； laser therapy

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