人體揮發性有機物及飲食導致呼氣丙酮變化之研究

An Investigation on Volatile Organic Compounds from Human Body and Diet Influence on Breath Acetone

李宜庭(none)

指導教授 ： 呂家榮

國立臺灣師範大學/理學院/化學系/碩士(2020年)

https://doi.org/10.6345/NTNU202000870

摘要

人體的代謝物一定程度上的反應了人體的生體狀態，而近幾年來，越來越來多學者在人體的揮發性有機化合物(VOCs)上進行了研究，其中，呼出氣體的分析為大宗。由於它的非侵入性和方便採樣的特點，使它具有在不同領域下運用的潛能。
本論文主要是研究並排除外界的干擾，開發人體VOCs的採樣方式，並利用自行組裝的前濃縮系統進行分析物的收集，以氣相層析質譜儀來進行分析。本研究鑑定出50種以上在人體呼出氣體中的化合物，同時配合環境的檢測，比對呼出氣體和環境氣體的相關性。發現呼出氣體中烯類、醇類、酮類和含硫化合物較環境濃度高，而其中含硫類化合物主要來自飲食。而烷類、醛類、酯類、含苯化合物則是環境中含量較高。而在手心皮膚表面能夠偵測出15種左右的的化合物。另外，利用實驗室之前開發的微氣相層析儀能夠快速簡便的達成特定化合物的連續分析，在本研究中用來檢測不同飲食對人體呼氣丙酮濃度隨時間變化的趨勢，對五位健康的受試者進行測試，發現呼出丙酮的濃度會隨著攝取的食物而發生改變，它能一定程度上的反應人體的代謝狀態。

關鍵字

人體揮發性有機物 ； 呼氣丙酮 ； micro-GC ； 非侵入式檢測

並列摘要

One’s metabolites can reflect the state of himself in certain degree. In recent year, more and more researchers have conducted the research in human’s volatile organic compounds (VOCs), especially in breath analysis. Because its non-invasion and convenience, make it full of the potential in different area.
This thesis aims to study and eliminate the interference when collecting the breath sample, develop a new method to sample human’s VOCs and collect the analytes by self -assembly preconcentrate system, analyze by Gas chromatography–mass spectrometry. With the method in this research, over 50 compounds in human breath can be detected. Also, by detecting the environment air to compare with the breath component. In these compounds, alkanes, alcohols, and ketones are found with higher concentration in human breath, whilst alkanes, aldehydes, esters and aromatic compounds have higher concentration in environment. In hand palm VOCs analysis, about 15 compounds can be found. Meanwhile, the influence of food intake on breath acetone was analysis by lab-made micro-GC, which can be used to analyze certain compound continuously. By analyzing five healthy subjects, finds out that the concentration of breath acetone changes after the food intake, which means breath acetone can reflect the metabolism in human in a certain way.

並列關鍵字

human VOCs ； breath acetone ； micro-GC ； non-invasive test

參考文獻

1. Dweik, R. A.; Amann, A., Exhaled breath analysis: the new frontier in medical testing. J. Breath Res. 2008, 2 (3), 030301.

2. Taalman, R. D., Isoprene: background and issues. Toxicology 1996, 113 (1-3), 242-246.

3. Haze, S.; Gozu, Y.; Nakamura, S.; Kohno, Y.; Sawano, K.; Ohta, H.; Yamazaki, K., 2-Nonenal newly found in human body odor tends to increase with aging. J. Invest. Dermatol. 2001, 116 (4), 520-524.

4. Dormont, L.; Bessiere, J. M.; Cohuet, A., Human skin volatiles: a review. J. Chem. Ecol. 2013, 39 (5), 569-578.

5. Wisthaler, A.; Weschler, C. J., Reactions of ozone with human skin lipids: sources of carbonyls, dicarbonyls, and hydroxycarbonyls in indoor air. Proc. Natl. Acad. Sci. U. S. A. 2010, 107 (15), 6568-6575.