利用溶膠凝膠法製備Ag/SiO2核殼奈米粒子及其抗菌應用之研究

Study of Ag/SiO2 core-shell nanoparticles prepared by Sol-Gel process on antibacterial application

吳念勳(Nien-Hsun Wu)

指導教授 ： 閔庭輝

國立虎尾科技大學/電機資訊學院/光電與材料科技研究所/碩士(2010年)

摘要

實驗中是以十六烷基三甲基溴化銨作為界面活性劑，以維生素C作為還原劑，還原前驅物硝酸銀而得到奈米銀微粒；鹼性環境下銀離子還原較佳，利用溶膠凝膠法將矽酸乙酯和乙醇混合反應生成二氧化矽，形成具核殼結構之銀/二氧化矽核殼奈米粒子。
紫外光/可見光光譜儀量測結果，當硝酸銀濃度從0.05M至0.2M改變，吸收峰由420nm移至409nm。由穿透式電子顯微鏡觀察的結果，發現約在PH值為10可清楚觀察到形成銀/二氧化矽核殼奈米粒子。透過EDX量測可證明所製備的核殼奈米粒子，分析出樣品的成分為Ag、C、O、Si。X射線繞射儀 (XRD)分析，在2θ=38.15°、44.35°、64.45°、77.53°出現Ag的特性峰。
在最低抑菌濃度的抗菌實驗顯示對於革蘭氏陰性菌和革蘭氏陽性菌，菌液約109CFU/ml都有抗菌及殺菌效果，即使將銀/二氧化矽核殼奈米粒子放置一星期後，仍具有相同效果。
銀奈米粒子外層由於有二氧化矽均勻包覆之保護下，透過非晶矽的二氧化矽層慢慢釋放銀離子，減少和空氣的接觸而氧化，達到延長抗菌的時間，使其表現與僅單純奈米銀粒子完全不同的性質，並且可藉由此技術將昂貴的材料包覆在價廉的物質中，表現所需的功能且節省大量的原料成本。

關鍵字

核殼 ； 溶膠凝膠法 ； 奈米粒子

並列摘要

In the preparation, we used Hexadecyltrimethyl ammonium bromide as surfactants, and the utility of surfactants could deal with the surface of nanoparticles. We made AA as a reducing agent, and that changed AgNO3 became nano Ag particles. In alkaline condition was good to reduce for nano Ag particles, we mixed the Tetraethyl orthosilicate and Ethanol to become SiO2 by using sol-gel method. The SiO2 were successfully coated with the nano Ag particles.
From UV/Visible results, while the concentration of AgNO3 was changed from 0.05M to 0.2M, and the peak of absorbance was shifted from 420nm to 409nm. TEM results indicate that in the PH value about 10, and to form Ag/SiO2 core-shell nanoparticles. EDX results prove that the elements of the core-shell nanoparticles were analyzed of Ag, C, O, Si. XRD results certificate that the peak were all Ag at 2θ=38.15°, 44.35°, 64.45°, 77.53°.
The minimal inhibitory concentration (MIC) antibacterial test exhibited antibacterial efficiency against both Gram positive bacterium and Gram negative bacterium, and the bacterium were about 109CFU/ml. Even we put the Ag/SiO2 core-shell nanoparticles in the air for a week.
As a result of that nano Ag particles under the protection of silica shell uniformly, and release the silver ions slowly to avoid oxidation directly. The time of antibacterial efficiency was extended. The Ag/SiO2 core-shell nanoparticles were different from the nano Ag particles characteristic. The core-shell structure nanoparticles, and expensive materials were coated with cheap ones. It not only increase functions, but also cost saving.

並列關鍵字

core-shell ； sol-gel processing ； nanoparticle

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