- 學位論文
醬油粕與芝麻粕添加對北蟲草固-液態培養生成生物活性物質產量影響之探討
Effect of Addition of Soy Sauce Cake and Defatted Black Sesame Meal on Production of Bioactivity Compounds from Cordyceps militaris in Solid-Liquid Cultivation
摘要
本研究利用已萃取過花青素之紫米為主要基質，進行醬油粕與芝麻粕添加對北蟲草固-液態培養所生成生物活性物質(蟲草素、腺苷、蟲草酸及蟲草多醣)產量影響之探討，其中固態培養基質為100％：0％、75％：25％、50％：50％、25％：75％、0％：100％等5種不同比例之紫米與醬油粕或紫米與芝麻粕的組合，並在22℃與70%相對濕度下，以固-態態培養方式北蟲草60天。實驗結果顯示，僅有未含有任何粕之100%紫米組、含25％醬油粕之紫醬組及含25％芝麻粕之紫芝組等三組形成子實體，其子實體中蟲草素含量為含25％芝麻粕之紫芝組最高；腺苷、蟲草酸及蟲草多醣含量則為含25％醬油粕之紫醬組最高，由此可知醬油粕與芝麻粕添加有助於提高北蟲草子實體中蟲草素與腺苷含量，但僅有醬油粕添加有助於提高子實體中蟲草酸與蟲草多醣含量；然而，若兩者添加量超過25%含量則抑制北蟲草生成子實體。固態培養基中所含有最高蟲草素產量為50％芝麻粕之紫芝組，含有最高腺苷含量為50％醬油粕之紫醬組；但含有最高蟲草酸含量則為100％紫米組；除了100％芝麻粕組之蟲草多醣含量較低外，其餘組之含量並無明顯差異性。
並列摘要
After anthocyanins extraction, the extracted purple rice was used as the main solid medium for Cordyceps militaris cultivation in this study. The effect of addition of soy sauce cake and defatted black sesame meal on production of bioactivity compounds (cordycepin, adenosine, cordycepic acid and polysaccharides) from C. militaris in solid-liquid cultivation for 60 days at 22oC and 70% RH (relative humidity) was investigated. The mixture ratios of the solid media of purple rice (PR) and soy sauce cake (SSC) or PR and defatted black sesame meal (DBSM) were set as 100%：0%, 75%：25%, 50%：50%, 25%：75%, 0%：100% (defined as 100%PR (or PR), 25%, 50%, 75%, 100%SSC or DBSM, respectively). The results showed that C. militaris could grow to form its fruiting body only cultivated in the solid media of 100%PR, 25%SSC and 25%DBSM (redefined as PR-SSC and PR-DBSM, respectively). The best cordycepin productivity from the fruiting body indicated its order for C. militaris cultivated in the media PR-DBSM. The yield of adenosine, cordycepic acid and polysaccharides of the fruiting body showed its order for C. militaris cultivated in the media PR-SSC. This indicated that the addition of SSC and DBSM could improve the productivities of cordycepin and adenosine from the fruiting body of C. militaris, but just only the addition of SSC could improve the yields of cordycepic acid and polysaccharides of the fruiting body. However, the formation of the fruiting body was totally inhibited when the addition of SSC or DBSM was more than 25%. The highest yields of cordycepin, adenosine and cordycepic acid from the solid media indicated that C. militaris mycelia cultivated in the media 50%DBSM, 50%SSC and 100%PR, respectively. The yields of polysaccharides from the solid media showed that there was no significant difference for C. militaris mycelia cultivated in all the solid media except for 100%DBSM with the less yield effect.