壹、越南產無柄紫靈芝子實體固醇類與三萜類化合物研究 貳、越南產糙葉花椒葉部化學成分研究

1. Steroids and Triterpenoids from the Fruiting Bodies of Ganoderma mastoporum Collected in Vietnam 2. Chemical Constituents from the Leaves of Zanthoxylum scabrum Collected in Vietnam

林啟文(Chi-Wen Lin)

指導教授 ： 郭賓崇

國立虎尾科技大學/文理學院/生物科技研究所/碩士(2013年)

摘要

由越南取得的無柄紫靈芝（Ganoderma mastoporum）子實體甲醇粗萃物，經由各種層析方法進行分離純化，共鑑定出8個固醇類與三萜類化合物。進一步針對純化所得的化學成分進行DPPH自由基清除與亞鐵離子螯合活性的測試，評估其抗氧化的能力。純化所得化合物將進一步送測藥理活性，藉此開發新的保健食品與抗發炎天然化合物。
糙葉花椒（Zanthoxylum scabrum）為芸香科植物，主要分布於越南北部、泰國東北部及中國南方部分地區。由越南採集的糙葉花椒葉部之甲醇粗萃物，經初步藥理活性篩選發現具有抗發炎活性。糙葉花椒葉部之甲醇粗萃物氯仿分畫層及不溶物層對fMLP/CB誘導人類嗜中性白血球超氧陰離子的生成具有抑制活性，其 IC50 分別為 2.79±0.34 及 2.20±0.08 μg/mL；對fMLP/CB誘導人類嗜中性白血球彈性蛋白酶的釋放亦具有抑制活性，其 IC50 分別為 1.97±0.27 及 2.63±0.18 μg/mL。因此針對生物活性畫分進行成分研究，目前由氯仿分畫層分離得到11個化合物。純化所得化合物將進一步送測藥理活性，希望藉此開發新的天然抗發炎藥物。

關鍵字

無柄紫靈芝 ； 三萜類 ； 保健食品 ； 糙葉花椒 ； 抗發炎

並列摘要

The methanol extracts from the fruiting bodies of Ganoderma mastoporum collected from Vietnam was purified to afford 8 compounds. To evaluate their in vitro antioxidant potentials, the purified compounds were examined for the scavenging activities of DPPH free radicals and capability of chelating ferrous ions. These isolates would be subjected into the pharmacology activity examinations to explore new natural anti-inflammatory drugs and may be potential sources for the development of healthy foods.
Zanthoxylum scabrum is a Rutaceae plants distributed in North Vietnam, Northeast Thailand and Southern China. In our studies the methanol extracts of the leaves of Z. scabrum from Vietnam screened for pharmacology activity has been found in vitro anti-inflammatory activity. The chloroform layer and precipitation layer of methanol extracts of the leaves of Z. scabrum inhibited fMLP/CB induced superoxide anion generation in a concentration-dependent manner with IC50 values of 2.79±0.34 and 2.20±0.08 μg/mL; inhibited fMLP/CB induced elastase release in a concentration-dependent manner with IC50 values of 1.97±0.27 and 2.63±0.18 μg/mL, respectively. Therefore, the present research is aimed to the bioactivity and the chloroform layer of methanol extracts of the leaves of Z. scabrum were fractionated and further purified to afford 11 compounds. These isolates would be subjected into the pharmacology activity examinations to explore new natural anti-inflammatory drugs.

並列關鍵字

Ganoderma mastoporum ； triterpenoids ； healthy foods ； Zanthoxylum scabrum ； anti-inflammatory

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