漸層氮化鈦鋯與鈦鋯非晶碳複合鍍膜之製備與生醫應用研究

Biomedical Application and Preparation of Gradient TiZrN and TiZr-amorphous Carbon Coatings

劉家旭(Jia-Xu Liu)

指導教授 ： 張銀祐

國立虎尾科技大學/工程學院/機械與電腦輔助工程系碩士班/碩士(2014年)

摘要

鈦及其合金在牙科診所與整形外科診所是常被用使用的人工關節和植入物，由於其具良好的生物相容性與機械性質，植入物和植入物表面形貌的機械性質與材料設計皆會影響治療效果。透過表面改質技術可改善植入物材料表面細胞的附著與擴散，而多項研究證實碳薄膜具有良好的生物相容性與機械性質。本研究使用陰極電弧沉積系統(Cathodic Arc Evaporation System, CAE)，使用之靶材有純Ti與純Zr，透過製程控制製備三種不同成分比例之TiZrN、TiZrCN、TiZr-amorphous Carbon三種薄膜沉積在Ti基材上。再分別由金黃色葡萄球菌(Staphylococcus aureus, S.a)、伴放線菌放線桿菌(Actinobacillus actinomycetemocomitans, A.a)與人體皮膚纖維母細胞(human skin fibroblast, SKF)測試薄膜之抗菌性與生物相容性。

　　藉由能量散佈光譜儀(Energy Dispersive Spectrometer, EDS)分析薄膜之化學成分組成，薄膜之晶相微結構與鍵結態採用X光繞射儀(X-ray Diffraction, XRD)、X光電子光譜儀(X-ray Photoelectron Spectroscope, XPS)、場發射掃描式電子顯微鏡(Field Emission Scanning Electron Microscope, FE-SEM)、穿透式電子顯微鏡(Transmission Electron Microscope, TEM)分析，藉由表面輪廓儀量測薄膜表面粗糙度，分別由百格測試與電位腐蝕量測薄膜附著性能和抗腐蝕性能。同時以體外MTT分析細胞活性，人體皮膚纖維母細胞(SKF)觀察薄膜對細胞貼附形態影響以及生物相容性。

　　研究結果顯示TiZrCN薄膜之C-H鍵結含量與乙炔流量成正比，C2H2：N2反應氣體流量比= 1:1之TiZrCN(1:1)薄膜試片為本次實驗中具最多C-H鍵結含量之薄膜，抗菌性能最佳，其次為TiZrCN(2:8)。同時，在碳膜中加入氮元素會使表面粗糙，使接觸角減小，使試片TiZrCN(2:8)與TiZrCN(1:1)在所有試片中較趨近於親水性質。MTT細胞活性測試結果也顯示TiZrCN薄膜之純鈦基材的SKF細胞活性最佳，且細胞貼附形貌最接近扇形擴散，為本研究中具有最適合做為生醫材料性質之薄膜。

關鍵字

薄膜 ； 氮化鋯鈦 ； 氮碳化鋯鈦 ； 非晶碳 ； 生物相容性

並列摘要

Titanium and titanium alloys have been used as artificial joints and implants in clinic of orthopedic and dental because of their good properties of biocompatibility and mechanical performance. Recently, surface modification technology has been adopted to improve adhesion and proliferation of cells on implant surface, and a number of studies have confirmed amorphous carbon films can improve biocompatibilities and mechanical properties of titanium. In this study, pure titanium and zirconium targets were used to deposit TiZrN、TiZrCN、TiZr-amorphous Carbon coatings with different chemical contents on titanium substrates using a cathodic arc evaporation system. Staphylococcus aureus (S. a)、Actinobacillus actinomycetemocomitans (A.a ) antibacterial analyses and human skin fibroblast(SKF) biocompatibility of the deposited coatings were conducted.
Characterize the composition of coatings was measured by energy dispersive spectrometer(EDS). The crystalline structures and bonding states of coatings were analyzed by X-ray diffraction(XRD), X-ray photoelectron spectroscope(XPS), field emission scanning electron microscope,(FE-SEM),transmission electron microscope(TEM). A surface profilometer was used to measure the surface roughness of the coatings. Potential resistance tests were used to measure the corrosion resistance. In vitro MTT analyses were used for cell activity identification. SKF cell adhesion morphology was also observed by FE-SEM.
The results show that the deposited TiZrCN coatings are hydrophilic because of lower contact angles. These TiZrCN coated Ti samples have the best antibacterial performance and SKF compatibility, and they are suitable for biomedical applications.

並列關鍵字

Coating ； TiZrN ； TiZrCN ； amorphous Carbon ； biocompatibility

參考文獻

1. Paul K. Chu, 2007, ” Plasma surface treatment of artificial orthopedic and

cardiovascular biomaterials”, Surface & Coatings Technology, Vol.201,

modification of biomaterials”, Journal of Materials Science and Engineering R, Vol.36, pp.143-206.

3. Rana N.S. Sodhi, 1996, ”Application of surface analytical and modification techniques to biomaterial research”, Journal of Electron Spectroscopy and Related Phenomena, Vol.81, pp.270-271.

4. Marc Long, H.J. Rack, ”Review Titanium alloys in total joint replacement–a materials science perspective”, Biomaterials, Vol.19, pp.1621–1639.