Optical coherence tomography (OCT) is an optical signal acquisition and processing method, with characteristics of a non-invasive, high-resolution and high sensitivity. Usually near infrared light measurements. In the study, the OCT system is based Michelson interferometer architecture. The different optical sources are used to the OCT system. Also, we compare the measured results with different optical source and the same sample. According to the obtained results, we can decide the best optical source in the OCT system.