- 學位論文
NRC-03 胜肽修飾多巴胺/還原氧化石墨烯奈米材料於乳癌近紅外光治療
Photothermal therapy Response of Breast cancer cells to NRC-03 peptide conjugated Dopamine –Modified Reduced Graphene Oxide under NIR illumination
摘要
近年來，乳癌一直是導致女性致病及致死率提高的主要原因之一。因此，發展高效率之乳癌治療方法確實具有其迫切之需求性。胜肽療法已經被應用於各種疾病之治療，尤其是在癌症治療領域具有極佳之發展潛力。許多具備抗菌活性之胜肽，同時也被發現具有抑制特定癌細胞生長之能力。然而，部分胜肽在生物系統中非常容易被分解。為解決此一限制發展因素，胜肽治療結合奈米科技於癌症治療之應用，越來越受到相關癌症治療研究領域之關注。目前奈米胜肽治療技術仍處於早期研究階段，有待更多研究能量之投入。本研究之目的為結合胜肽和奈米光熱技術進行新型乳癌治療技術之研發，研究中將結合NRC-03,多巴胺和氧化石墨烯合成出具備低生物毒性之NRC-03- pDA / rGO奈米藥物，並進一步評估該奈米藥物對MCF-7乳癌細胞之殺傷效率。實驗結果顯示，由於NRC-03- pDA / rGO具備極佳近紅外光吸光效率及光熱轉換效率，因此其可以有效的對乳癌細胞產生較高的殺傷作用。此外，將NRC-03修飾於pDA/rGO表面確實可以提高NRC-03胜肽於生物系統之穩定性。本研究亦證實NRC-03- pDA / rGO結合了NRC-03，多巴胺和rGO的優點，同時具備極佳之生物相容性和抗乳癌細胞能力，顯示NRC-03- pDA / rGO於乳癌之治療具有極高之發展潛力
並列摘要
Breast cancer remains the leading causes of high morbidity and mortality among women. Therefore, there is still an urgent need to develop effective breast cancer treatment. Peptide-based therapy has been applied in various diseases, particularly for cancer treatment. The antimicrobial peptide ability can inactivate a wide range of cancer cells. However, some peptides are easily biodegradable in biological system. Under this limitation, peptide therapy based on nanotechnology has received increased attention. Peptide-based nano-therapeutic therapy is a new type of cancer treatment that is still in the early stages of research. The objective of this study was to synthesis a nanocomposite, NRC-03 peptide conjugated polydopamine-modified reduced graphene oxide nanocomposite, to facilitate treatment of breast tumor cells by near-infrared (NIR) light-activatable photothermal therapy. Our results show that immobilization of NRC-03 on the surface of dopamine-modified rGO can increase the stability of NRC-03 peptide in biological system. Furthermore, the results also show that our NRC-03-pDA/rGO, which combines the advantages of NRC-03, dopamine and rGO, displays excellent biocompatibility and anti-tumor cell ability, suggesting great potential for application in the photothermal therapy of breast cancer.