- 學位論文
利用表面電漿共振感測器檢測細菌濃度
Using Surface Plasmon Resonance Sensor for Detecting Bacterial Concentration
摘要
表面電漿共振感測技術是一種快速、即時性、且操作方便無需標定就能檢測生物分子之間的交互作用，被廣泛應用於醫療、食品、環境等檢測領域。表面電漿共振感測器藉由偵測金屬薄膜表面上的折射率變化進而使入射光共振角度改變來分析生物分子之間的交互作用。 本研究利用表面電漿共振感測技術來進行細菌濃度檢測，以固定入射光的波長檢測不同種細菌及其濃度，藉由檢測共振角度變化得知待測分析物濃度改變的情況，並建立表面電漿共振角度與細菌濃度之間的關係曲線。另外配合平板塗抹法的菌落數測定，將不同細菌濃度以無菌水做連續稀釋，計算其不同稀釋倍數形成之菌落數，並從菌落數推算該細菌濃度。 最後，利用表面電漿共振角度與菌落數之間的關係進行快速檢測細菌濃度。未來可利用表面電漿共振感測器快速檢測細菌濃度取代傳統細菌檢測法中耗時的平板塗抹法之菌落數測定。
並列摘要
Surface plasmon resonance sensing technique is a rapid, real-time, convenient operation without between calibrations can detect biomolecular interaction, is widely used in the field of medical, food, environment detection. Surface plasmon resonance sensor by refraction detection of metal film on the surface of a rate change so that the incident light resonance angle change to analyze the interaction between biological molecules. In this study, using surface plasmon resonance sensing technology to carry out bacterial concentration detection, at a fixed wavelength of the incident light detection of different concentration, the detecting resonance angle can change that change the object to be measured, and establish the relationship between the surface plasmon resonance angle and the bacterial concentration. Also with the determination of colonies number application method, spread plate, the different concentration with sterile water for dilution, colonies forming the dilution of the number, and to calculate the concentration of the bacteria from the number of colonies. Finally, based on the relation between the surface plasmon resonance angle and the number of colonies for rapid detection of bacterial concentration. Using of surface plasmon resonance sensor for rapid detection of bacterial concentration to replace the traditional detection method time-consuming spread plate method in the determination of the number of colonies in future.