不同銀含量之氧化鉭薄膜對抗菌性能與生物相容性的影響

Antibacteriai Properties and Cytocompatibility of Tantalum Oxide Coatings with Different Silver Content

陳弘叡(Hung-Jui Chen)

指導教授 ： 張銀祐

國立虎尾科技大學/工程學院/機械與電腦輔助工程系碩士班/碩士(2015年)

摘要

除了鈦金屬以外鉭氧化物已被有效應用在骨骼、關節、牙科植入物等生物醫學方面，能有效提高植入物與人體的結合。本研究採用磁控濺鍍系統選用Ta靶及Ag靶並通以氧氣沉積五氧化二鉭(Ta2O5)薄膜及調整Ag靶功率得到Ta2O5-Ag20W及Ta2O5-Ag40W薄膜。對Ta2O5薄膜高溫退火800℃觀察期結晶情況再對Ta2O5-Ag薄膜進行350℃快速退火(RTA)處理使銀粒子析出薄膜表面，藉此獲得更好的抗菌性並探討機械性質及細胞生物相容性。
本實驗利用X光繞射儀(X-ray Diffraction,XRD)、場發射掃描式電子顯微鏡(Field-emission scanning electron microscope,FE-SEM)、穿透式電子顯微鏡(Transmission Electron Microscope,TEM)、原子力顯微鏡(Atomic Force Microscopy,AFM)、恆電位電流儀(Potentiostat/Galvanostat)觀察薄膜的表面形貌、結晶性、表面粗糙度及耐腐蝕能力。採用X光電子光譜儀(X-ray Photoelectron Spectroscope,XPS)、能量散布光譜儀(Energy Dispersive Spectrometer,EDS)分析薄膜元素組成、鍵結態及銀含量變化。抗菌性實驗藉由Syto9螢光染色法觀察金黃色葡萄球菌(Staphylococcus aureus)之細菌存活率。利用MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)試劑分析細胞活性以人類皮膚纖維細胞(CCD-966SK)觀察細胞形態、貼附狀況及生物相容性。
結果顯示Ta2O5-Ag薄膜經350℃快速退火後Ta2O5依然為非晶結構，需加熱至800℃才會開始結晶，但350℃快速退火後Ag已開始結晶並有Ag粒子析出薄膜表面的現象，Ag含量也由20W:3.03at.%增加至20W-RTA:5.93at.%及40W:10.61at.%增加至40W-RTA:13.68at%，發現Ag含量除了隨著Ag靶功率提高之外，快速退火後也會影響Ag含量。抗菌測試結果顯示隨著Ag含量增加可以有效提升抗菌能力，因此40W-RTA擁有最佳的抗菌能力，但也因Ag具有微量毒性故40W-RTA在生物相容性表現較差。生物相容性及抗腐蝕性能則以Ag-20W有最好的表現，綜合以上各項實驗結果得到Ta2O5-Ag20W為最佳製程參數。

關鍵字

氧化鉭 ； 磁控濺鍍 ； 薄膜 ； 生物相容性 ； 抗菌性 ； 銀

並列摘要

Tantalum oxides and their coatings have been proved to increase their applications in the biomedical fields by improving their osseointegration and wear resistance. In this study, doped tantalum oxide coatings containing different proportions of Ag were deposited on SS304 materials. A twin-gun magnetron sputtering system was used for the deposition of the tantalum oxide-Ag coating. In this study, S. aureus are Gram-positive and Gram-negative bacteria, respectively, that exhibit physiological commensalism on the human skin, nares, and mucosal and oral areas. Both bacteria were chosen as the model for in vitro anti-bacterial analyses by a fluorescence staining method employing Syto9. The cytocompatibility and adhesive morphology of human skin fibroblast cells (CCD-966SK) on the coatings were also determined by using the MTT assay. It showed that Ta2O5 and Ta2O5-Ag composite coatings not only exhibit improved antibacterial effects against S. aureus and A. actinomycetemcomitans but also had good SKF cell cellular biocompatibility.
Characterize the composition of coatings was measured by energy dispersive spectrometer(EDS). The crystalline structures and bonding states of coatings were analyzed by X-ray diffraction(XRD), X-ray photoelectron spectroscope(XPS), field emission scanning electron microscope,(FE-SEM),transmission electron microscope(TEM). Atomic force microscope(AFM) was used to measure the surface roughness of the coatings. Potential resistance tests were used to measure the corrosion resistance. In vitro MTT analyses were used for cell activity identification.
The results show that the deposited Ta2O5 coated SS304 materials have the best antibacterial performance and they are suitable for biomedical applications.

並列關鍵字

tantalum oxide ； silver ； human skin fibroblast ； thin film ； nano structure ； antibacterial

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