蜂子粉對人工蛹蟲草子實體之抗氧化活性分析

Bee pupa powder on the antioxidant activity of artifical culture of the fruiting body of Cordyceps militaris

蔡博宇(Bo-Yu Cai)

指導教授 ： 楊源昌

國立虎尾科技大學/文理學院/生物科技研究所/碩士(2011年)

摘要

近年來，具有改善身體機能的各種保健食品日漸受到民眾的重視，期
能藉由保健食品來達到預防疾病之發生。蛹蟲草別名北冬蟲夏草、北蟲草，因具有獨特的化學組成，所以具有醫療保健效果。本實驗目的為以人工蛹蟲草固態培養並搭配九種氮來源(黃豆粉100%、蜂子粉25%、蜂子粉50%、蜂子粉75%、蜂子粉100%、蠶蛹粉25%、蠶蛹粉50%、蠶蛹粉75%、蠶蛹粉100%)的水萃取物之體外抗氧化性及其生理活性。結果顯示當Cordyceps
militaris BCRC32219以蠶蛹粉25%當氮源時，其總多酚含量最高( 1043.30 ± 14.58 ppm, as gallic acid )；C. militaris LS901以蜂子粉75%當氮源，其總多酚含量為808.87 ± 15.43 ppm ( as gallic acid )，效果最好；而C. militaris
LS902以蜂子粉100%當氮來源時，其總多酚含量達最高( 828.74 ± 14.75 ppm, as gallic acid )。蟲草素含量方面，此三種菌株以黃豆粉100%當氮來源時，其子實體蟲草素含量皆顯著高於其它九種氮來源。而體外抗氧化試驗中，此三種菌株皆具抗油脂過氧化物、清除DPPH自由基能力、螯合亞鐵金屬離子能力、還原能力等抗氧化特性。此三種菌株之各萃取液其螯合亞鐵金屬離子能力及還原能力皆隨萃取物濃度之增加而增加。於抗油脂過氧化能力，三種菌株之各萃取液均相當於人工合成之抗氧化劑BHT。清除DPPH自由基能力方面，此三種菌株之各萃取液在濃度 3 mg/ml 時清除率可達80%以上。上述實驗結果顯示此三種菌株子實體之萃取物具有抗氧化特性，且不同萃取物之抗氧化能力不盡相同。

關鍵字

蛹蟲草 ； 總多酚 ； 蟲草素 ； 抗氧化性

並列摘要

Recently, there is increasing interest in a variety of bodily functions to improve the health food by the people, which achieved through health food to prevent disease occurrence. Cordyceps militaris, as Cordyceps, has a unique chemical composition, and therefore have a health effect. This study was aimed to evaluate the vitro-antioxidant activities and its physiological activitues of the water extracts from artificial cultured Cordyceps militaris with nine nitrogen source (100% soybean meal, 25% bee pupa powder, 50% bee pupa powder, 75% bee pupa powder, 100% bee pupa powder, 25% silkworm pupa powder, 50% silkworm pupa powder, 75% silkworm pupa powder, 100% silkworm pupa powder,) . The results showed that when C. militaris BCRC32219 with the nitrogen source of 25% silkworm pupa powder, the highest amount of total phenolics (1043.30 ± 14.58 ppm, as gallic acid); C. militaris LS901 the nitrogen source of 75% bee pupa powder, the amount of total phenolics was
808.87 ± 15.43 ppm (as gallic acid), which was the greatest ; and C. militaris LS902 with the nitrogen source of 100% bee pupa powder , the highest amount of total phenolics (828.74 ± 14.75 ppm, as gallic acid). The greatest amount of Cordycepin in these three strains with the nitrogen source of 100% soybean powder. The in vitro antioxidative activities assays include anti-lipid peroxide fields, DPPH radical scavenging effect, ferrous metal ion chelating power, reducing power; the all extracts of three strains obtained their abilities depended strongly on their concentrations at the ferrous metal ion chelating power and reducing power. All the extracts exhibited lipid peroxidation which were equivalent to the BHT. The all extracts reached more than 80% of DPPH radical scavenging effect at high concentrations 3 mg/ml. These results showed that all extracts obtained antioxidative abilities.

並列關鍵字

Cordyceps militaris ； total phenolic content ； cordycepin ； antioxidative

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