相移干涉表面電漿共振系統於生化檢測應用

Application of Phase-Shift Interferometry Surface Plasmon Resonance System in Bio-chemical Detection

林芃志(Peng Zhi Lin)

指導教授 ： 郭文凱

國立虎尾科技大學/電機資訊學院/光電與材料科技研究所/碩士(2013年)

摘要

本文主要研究表面電漿共振(surface plasmon resonance)技術應用分析生物分子間的交互作用，實驗架構使用相移干涉表面電漿共振影像系統(Investigation of Phase-Shift Interferometry Surface Plasmon Resonance Imaging System)來偵測相位變化。利用Labview自動化程式結合液晶控制器(Liquid Crystal Device Controller)、外接式網路攝影機(Web Cam)及電腦做自動化的影像擷取，於玻璃基板上方以熱蒸鍍法鍍上一層金(Au)，再藉由匹配油附著於稜鏡上方，即成為稜鏡耦合表面電漿共振感測元件，實驗中使用牛血清蛋白(Albumin from bovine serum)與兔抗牛血清蛋白抗體(Anti-Bovine Albumin antibody produced in rabbit)量測其反應結果，本實驗架構可量測大範圍的生物樣品資訊，實驗中將量測區域分為有固化抗原的區域與沒有固化抗原的區域，經由抗體反應並比較抗原-抗體反應前後的相位變化。

關鍵字

表面電漿共振 ； 相移干涉 ； 牛血清蛋白

並列摘要

This paper mainly studies surface plasmon resonance technology application to analyze interaction between biological molecules. The experimental framework uses Phase-Shift Interferometry Surface Plasmon Resonance Imaging System to detect phase change. It utilizes an Labview automated program combined with a Liquid Crystal Device Controller, an external Web Cam and a computer to conduct automated image capture, has a layer of Au plated on glass substrate via thermal evaporation, and attaches it to the upper part of a prism through matching liquid, finally forms a prism coupling surface Plasmon resonance sensing component. In the experiment, Albumin from bovine serum and Anti-Bovine Albumin antibody produced in rabbit are used to measure reaction results. The experimental framework can measure a wide range of biological sample information. The measuring area is divided into areas with and without curing antigen in the experiment, which are taken antibody reaction, meanwhile, phase changes before and after antigen-antibody reaction are compared.

並列關鍵字

Surface Plasmon Resonance ； Phase-Shift Interferometry ； Albumin from bovine serum

參考文獻

[1] A.V. Kabashin, et al., 2009, “Phase and amplitude sensitivities in surface plasmon resonance bio and chemical sensing,” Opt. Express, 17(23), pp. 21191-21204 .

[2] P.I. Nikitin , et al., 1999 , “Surface plasmon resonance interferometry for biological and chemical sensing,” Sensors and Actuators B, 54, pp. 43-50.

[3] Pei, R., Yang, X., & Wang, E., 2001,“Enhanced surface plasmon resonance immunosensing using a streptavidin–biotinylated protein complex.” The Analyst 126 (1), 4-6

[4] Wegner, G. et al., 1998, “Real-time surface plasmon resonance imaging measurements for the multiplexed determination of protein adsorption/desorption kinetics and surface enzymatic reactions on peptide microarrays”, Biochemistry 37, 8735-8742.

[5] Kukanskis, K. et al., 1999,“Detection of DNA hybridization using the TISPR-1 surface plasmon resonance biosensor” Analytical biochemistry 274 (1), 7-17 .