水熱合成含氟磷酸鈣鍍層改善生物可降解性AZ80鎂合金之腐蝕阻抗與成骨細胞反應性研究

鎂合金質輕、彈性模數與人體骨骼相當接近，作為硬組織取代材料而植入人體後可自然降解以避免二次手術。然而鎂合金活性高，在水溶液環境下容易腐蝕，基於氟基磷灰石(FHA)具有良好生物相容性、生物活性及引導骨生能力，所以本研究藉由水熱法在175ºC、2小時、飽和蒸汽壓環境下，直接合成FHA鍍層並析鍍於AZ80鎂合金表面，目的為在水熱合成過程中藉由F─離子的部分取代，以獲得更佳之FHA鍍層特性，並藉由FHA鍍層改善AZ80鎂合金之抗腐蝕性與細胞反應性。研究結果顯示：在模擬體液環境下進行浸泡測試以及電化學腐蝕測試，浸泡32天後FHA鍍層依然具有保護鎂合金之特性，F離子取代使鍍層結構穩定。極化曲線結果顯示表面披覆FHA鍍層有效提高AZ80鎂合金抗腐蝕能力，相較於無摻雜F之HA鍍層以及AZ80鎂合金腐蝕電位顯著提高。體外細胞培養試驗後發現經過24小時之MG63骨細胞顯著增生並貼附於鍍層表面，顯示水熱法披覆FHA鍍層於AZ80鎂合金之生物反應性佳，為具有潛力的硬組織植入物材料。

關鍵字

氟基磷灰石；鍍層；水熱法；電化學腐蝕阻抗；細胞培養

並列摘要

Lightweight magnesium (Mg) alloys have a low elastic modulus, which value very close to the human bone. Biodegradable Mg alloys can be used for medical hard tissue implants in orthopedic applications without revision surgery. However, Mg alloys are notably characterized by low corrosion resistance in physiological solutions. On the basis of the excellent biocompatibility, bioactive and osteoconductive properties of fluorohydroxyapatite (FHA), a FHA surface coating was hydrothermally synthesized on the AZ80 Mg alloy at 175ºC under a saturated steam pressure atmosphere. The aim of this present study is not only to obtain an optimal FHA coating with the substitution of F─ ions, but improving corrosion resistances and cell responses of the AZ80 Mg alloy. Experimental results show that AZ80 Mg alloy can be protected by the FHA coating even though immersion in the simulated body fluid after 32 days. FHA coating stability is improved after the substitution of F─ ions. In addition, the corrosion resistance of AZ80 Mg alloy can be effectively improved by the deposition of FHA coating. In vitro cell culture results represent that significant proliferation and adhesion of MG63 cells on the FHA-coated AZ80 Mg alloy is demonstrated after testing for 24 hours. It is recognized that FHA-coated AZ80 Mg alloy shows a good biological reponses, and it can be considered as an attractive, excellent hard tissue substitute material for further biomedical applications.

並列關鍵字

fluorohydroxyapatite ； coatings ； hydrothermal ； corrosion resistance ； cell culture

參考文獻

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水熱合成含氟磷酸鈣鍍層改善生物可降解性AZ80鎂合金之腐蝕阻抗與成骨細胞反應性研究

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