微流道中細胞分離與移動之參數設計

微流體生物晶片藉由微機電系統的發展，將對於生醫科技領域有相當大的助益。本研究以離心力作為驅動力，使用Comsol分析軟體之對流體的粒子追蹤模組，模擬與觀察微粒子在微流道內移動之數值分析的情形。藉由PDMS翻模來獲得最佳的微流道幾何結構，再利用邊鞘流流體對樣品流的水力壓縮效果，探討改變細胞移動方向的影響。從模擬與實驗所獲得的結果，來證明我們設計一微流道幾何應用於生醫晶片上的可行性，做為流道幾何設計上的參考依據。由於當初發現沒有人使用粒子追蹤模組來進行模擬細胞的移動情形，因此我們就來試著做模擬。而細胞的特性與狀況不容易掌控，所以模擬與實驗的結果會有所差別。PDMS翻模不僅能降低成本，還能使其量產化，期盼能應用於生物醫學之相關領域。

關鍵字

離心力；粒子追蹤；微流道；邊鞘流

並列摘要

Microfluidic biochips developed by micro-electromechanical systems, biomedical science and technology will be there for a considerable help. In this study, the centrifugal force as the driving force, the use of the fluid analysis software Comsol particle tracking module, analog and observe particles move in a micro-flow channel of the numerical analysis of the situation. By PDMS molds to get the best micro-flow channel geometry, reuse edge sheath flow of hydraulic fluid in the sample stream compression effect, explore the impact of changing the direction of cell movement. From the simulation and experimental results obtained to prove that we have designed a microfluidic chip geometry for Biomedical feasibility, as the flow channel geometry design reference. Since no one had found using particle tracking module to simulate cell migration situation, so we have to try to do the simulation. The cell characteristics and situation is not easy to control, so the simulation and experimental results will be different. PDMS molds can not only reduce costs, but also to make mass production, hope can be used in biomedical related fields.

並列關鍵字

Centrifugal force ； Particle tracer ； Microchannel ； Sheath flow

參考文獻

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[12] 王宇宏(民99)，人類自然殺手細胞對血癌細胞毒殺特性研究之生物晶片設計與開發，國立臺灣大學應用力學研究所碩士論文。

[19] 鄭宜肪(民99)，介電泳式微流體晶片與其應用於生物微粒與分子之操控與檢測，國立成功大學奈米科技暨微系統工程系博士論文。

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國際替代計量

微流道中細胞分離與移動之參數設計

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