- 學位論文
熱擠製式三維列印技術製作骨組織工程支架與其骨髓間葉幹細胞體外反應之研究
Biofabrication of Bone Scaffold Using Thermal Extrusion Three-dimensional Printing and In-Vitro Response of Mesenchymal Stem Cell
摘要
本研究的目的是將利用熱擠製三維列印系統，自行合成的mPEG-PCL-mPEG高分子材料製成骨組織支架，並與添加二氧化鋯粉以及氫氧磷灰石所製成之骨組織支架，相互探討其對細胞活性與機械性質之差異性。藉由更改三維熱擠製列印系統的運動路徑，可以使骨組織支架側邊孔洞形成，增加支架的孔隙率，正面孔洞可維持450-500μm側邊孔洞高度可控制於200μm左右。自行合成的mPEG-PCL-mPEG高分子材料藉由MTT細胞活性實驗，進行1、3、5、7、9天的體外細胞培養，證明與骨髓間葉幹細胞(Mesenchymal stem cells，MSCs)進行體外細胞培養可達到細胞增生之趨勢，而添加二氧化鋯粉所製成之骨組織支架，經由抗壓測試以及硬度試驗得知，其不僅不會影響細胞生長，且增加骨組織支架的機械性質，並將氫氧磷灰石(HA)添加於骨組織支架中，更能使細胞增生之趨勢更為明顯。
並列摘要
The aim of this study was to investigate the difference between three kinds of bone tissue scaffolds on cell viability and mechanical properties by using thermal extrusion three-dimensional printing system to synthesize kinds of materials which are mPEG-PCL-mPEG polymer, Zirconia and Hydroxyapatte. By changing the moving path of thermal extrusion three-dimensional printing system can make the holes form in the side of bone tissue scaffolds and increase porosity on scaffold, holes in the front can be maintained in 450-500μm while holes in the side can be controlled at a height of about 200μm. After synthesized mPEG-PCL-mPEG polymer worked through MTT in-vitro cell culture assays for 1, 3, 5, 7, 9 days, the trend of cell proliferation can be proved. The scaffold for bone tissue that is added Zirconia has no impact on cell growth, and can increase mechanical properties of scaffold through the test of compression and hardness. The trend of cell proliferation can be more obvious after adding Hydroxyapatte to bone tissue scaffold.