開發梯度導模共振陣列感測晶片以應用於慢性腎臟病之快速篩檢

隨著人口老化，慢性病越發盛行，若能及早發現便能及早給予治療，因此快速又準確的檢測便顯得極為重要，故本研究的目標在於開發快速、低成本、高靈敏度之生物感測器。本研究應用週期梯度導膜共振開發一種感測器，利用生物分子鍵結後折射率改變的特性，從CCD上觀測光強度的分布圖，並由強度的偏移量來量測生物分子之濃度變化。為了驗證檢測生物分子的可行性，本研究以Albumin與Creatinine作為目標物進行量測，將導膜共振感測器結合微流道進行檢測，每次量測只需要60µl的檢體，Albumin與Creatinine的檢測極限分別為5.81 µg/ml與6.91µg/ml。綜合上述所說，我們成功驗證了週期梯度導膜共振檢測生物分子的能力，並憑藉著其較小的尺寸與簡單的光路設計，可以進一步將GGP-GMR與智慧手機結合，並應用於定點照護。

關鍵字

導膜共振；白蛋白；肌酸酐；慢性腎臟病

並列摘要

As the population ages, chronic diseases become more prevalent. Treatment can be prescribed in time if detected early; therefore, rapid and accurate detection is extremely important. The goal of this study is to develop rapid, low-cost, and sensitive biosensors. In this study, a sensor based on gradient grating period guided-mode resonance (GGP-GMR) was developed. The change in refractive index upon adsorption of biomolecule causes the shift in intensity distribution at a CCD. The amount of intensity shift can be correlated to the sample concentration. To demonstrate its biosensing capability, a GGP-GMR was integrated with microfluidic channel for albumin and creatinine measurement. Only 60 µl of samples were required to achieve limit of detection of 5.81 and 6.91 µg/ml for albumin and creatinine, respectively. In summary, the GGP-GMR was successfully demonstrated for biomolecule detection. With its compact size and simple optical path design, a GGP-GMR can be further integrated with a smartphone for potential point of care applications.