According to the requirement on the detection of display performance in thin film transistor liquid crystal display (TFTLCD), a multi-functional instrument for the measurement of voltage holding ratio (VHR), residual direct current (RDC), and electro-optical characteristics of liquid crystal cells have been developed by our research group. In order to expand the application field and measurement range for abovementioned parameters, in this study, a simple temperature-control system consisting of heating chips and cooling systems is designed and combined into our proposed instrument. We also integrate various types of circuits and whole framework for the instrument to obtain highly automatic measurement and numerical analysis through the data acquisition and interface of virtual instrument control. Furthermore, electrical properties such as the VHR and RDC, of twisted nematic and planar-aligned liquid crystal cells under specific measuring conditions are presented.