探討光照對Aspergillus ficuum NRRL3135在液態發酵中生產phytase之影響

探討光照對Aspergillus ficuum NRRL3135在液態發酵中生產phytase之影響

余程偉(Cheng-wei Yu)

指導教授 ： 徐敬衡

國立中央大學/工學院/化學工程與材料工程學系/碩士(2008年)

摘要

飼料添加劑phytase酵素加至飼料中，幫助禽畜類吸收金屬離子使用與提升飼料中磷含量的使用率，進而促進禽畜的消化率於產肉率提升。近年來眾多文獻提出同Aspergillus屬之Aspergillus nidulans 、Aspergillus fumigatus、Aspergillus parasiticus、Aspergillus flavus
為含有光接收器之真菌，因此藉由外界不同光源照度，影響菌體生長、產物代謝。
本研究主要目的是在探討光照對於同Aspergillus屬之Aspergillus ficuum NRRL3135生產phytase之影響。先由發酵槽實驗探討白光強度因著光照的距離而調整其照度，研究範圍從0、500、1000、1500、2000、2500 Lux；然而產物phytase活性則隨著照度的增加而隨之提升，達到最適化2000 Lux而後遞減；當操作條件為白光2000 Lux照度時，phytase活性為照度0 Lux時的6倍，比生長速率也提昇約1.93倍之多，然而productivity則提升了7.2倍之多。進而深入探討同光照度2000 Lux之不同光波長，探討範圍由LED Blue light(430 nm-530 nm)、LED Green light(480 nm-590 nm)至LED Red light(590 nm-670 nm)，產物phytase活性則隨著光波長降低而有所提昇，當光波長為LED Blue light(430 nm-530 nm) phytase活性為LED Red light(590 nm-670 nm)時的2.35倍，比生長速率也提昇約1.49倍，而productivity則是提升了2.54倍。綜合以上介紹，由光強度至不同光波長實驗，初步證實光照對於Aspergillus ficuum NRRL3135生產phytase均有所影響。

關鍵字

Aspergillus ficuum NRRL3135 ； phytase

並列摘要

Adding phytase into feed will help adsorption dietary minerals and increase the P adsorption on poultry,thus improve digestive of poultry.
In recent years, several study indicated fungus of Aspergillus nidulans、Aspergillus fumigatus、Aspergillus parasiticus、Aspergillus flavus are have photoreceptors, so light intensity factor is affecting biomass growth, formation of primary and secondary metabolites.
We are interested in realizing the effects of light intensity on growth biomass and production phytase of Aspergillus ficuum NRRL3135.In this study, we show that the light intensity effects on Aspergillus ficuum NRRL3135 of producing phytase.
In air-lift bioreactor, the white light intensity of lux was controlled by changed the distance of light source, worked in 0、500、1000、1500、2000、2500 lux, phytase activity was increasing with high lux, under optimal conditions,2000 lux, phytase activity was 6 times, specific growth rate was 1.93 times, productivity was 7.2 times at 2000 lux compared with 0 lux.
Studies in different wavelength in 2000 lux,the wavelength range from LED Blue light(430 nm-530 nm)、LED Green light(480 nm-590 nm) 、LED Red light(590 nm-670 nm), phytase activity was increasing with low wavelength,when LED Blue light(430 nm-530 nm), phytase activity was 2.35 times, specific growth rate was 1.49 times, productivity was 2.54 times compared with LED Red light(590 nm-670 nm).

並列關鍵字

Aspergillus ficuum NRRL3135 ； phytase

參考文獻

Mycophenolic acid之影響 國立中央大學化學工程與材料工程研究

立東海大學化學工程研究所 碩士論文

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