阻抗式生物感測器應用於人類白蛋白檢測之研究

The Research of Impedimetric Biosensors for Human Serum Albumin Detecton

薛凱鴻(Kai-Hung Hsueh)

指導教授 ： 蔡章仁

國立中央大學/資訊電機學院/電機工程學系/碩士(2008年)

摘要

本研究著重於開發一種阻抗式生物感測器應用人類白蛋白（human serum albumin，HSA）偵測。研究初期設計單一式及陣列式指叉電極兩種感測晶片，藉由微影製程技術實現，並透過LCR meter及電化學阻抗分析儀分析感測晶片的電容及阻抗特性。
在人類白蛋白捕捉檢測上，透過化學修飾的方法在晶片表面先固定一層自組單層膜，再以針對人類白蛋白具有高親和性的藍色染料Cibacron Blue F3GA（CB）作為捕捉不同濃度人類白蛋白的觸手。實驗結果發現，單一式比陣列式指叉電極感測晶片對於人類白蛋白捕捉上有較高的分辨度，約為6.7倍，且單一式指叉電極感測晶片可量測到的人類白蛋白濃度為0.1到0.4 mg/ml，與人類白蛋白被捕捉前後之阻抗改變量成一線性關係，其關係式為Y=6.13+546.78X，R2=0.997。
在本研究為了達到可攜式的人類白蛋白檢測方式，也建立一套微流道感測系統，包含電容偵測電路、微流道系統及LabVIEW監測畫面。在電容偵測電路的量測上，單一式指叉電極感測晶片可偵測到0.1到0.4 mg/ml之人類白蛋白濃度，與人類白蛋白被捕捉前後之直流電壓改變量成一線性關係，其關係式為Y=17.91+323.72X，R2=0.999。

關鍵字

人類白蛋白 ； 阻抗式生物感測器 ； 指叉電極

並列摘要

This research focused on developing an impedimetric biosensor for human serum albumin (HSA) detection. Two kinds of chips, unitary-type interdigitated electrode (u-IDE) and array-type interdigitated electrode (a-IDE) were designed in early study, both were made by microfabrication technology. The chips were measured by the LCR meter and electrochemical impedance analyzer (IM-6ex) to analyze the character of capacitance and impedance.
In HSA detection, the method of surface modification was based on self-assembled monolayer and the blue dye Cibacron Blue F3GA (CB) with high affinity for HSA was used as a receptor to capture the different concentrations of HSA. The experimental results showed that the resolution of HSA detection, the u-IDE was higher than the a-IDE about 6.7 times. The u-IDE can detect the concentrations of HSA between 0.1 and 0.4 mg/ml with IM-6ex. The impedance change of before and after HSA being captured by CB had a linear relationship with the concentrations of HSA. The regression equation was Y=6.13+546.78X with R2=0.997.
In order to achieve the portable HSA detection method, we also built a micro-fluid detection system. This system included the capacitance detection circuit, micro-fluid system and LabVIEW monitoring instrument. The u-IDE can detect the concentrations of HSA between 0.1 and 0.4 mg/ml with the capacitance detection circuit. The DC voltage change of before and after HSA being captured by CB had a linear relationship with the concentrations of HSA. The regression equation was Y=17.91+323.72X with R2=0.999.

並列關鍵字

interdigitated electrode ； human serum albumin ； impedimetric biosensor

參考文獻

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