奈米矽質譜晶片於質譜檢測之應用研究

The research of nanostructured silicon chip in mass spectrometry analysis

陳崑漢(Kun-han Chen)

指導教授 ： 曹嘉文

國立中央大學/工學院/機械工程學系/碩士(2011年)

摘要

本研究以奈米矽（nanostructured silicon, nSi）作為質譜晶片之分析基材，吾稱此方法為nSi-MS。nSi-MS無需基質輔助以及高靈敏度的特點，特別適合小分子分析物的分析；並且，其簡易前處理以及樣本量需求少的優勢能使分析流程僅在15分鐘內即可快速完成。
於研究中，我們針對nSi-MS質譜檢測平台，首先進行樣本前處理以及樣本分離的實驗測試，其中包括了尿液檢體雜質的前處理、混合生肽樣本的分離以及等電位電泳轉置技術的整合，評估nSi質譜晶片進行簡易前處理的表現及效能。
並且，我們進一步利用nSi質譜晶片的高靈敏度特點，針對目前濫用問題嚴重的多種小分子濫用管制藥品進行實驗，實驗樣本同時包括了藥用標準品及毒癮者的真實尿液檢體，共有安非他命、甲基安非他命、可待因、嗎啡、愷他命及MDEA等六種小分子藥物，並對其檢測線性(Linearity)、檢測靈敏度(Limit of detection)、檢測重複性(Repeatibility)及定量分析(Quantification)等表現進行討論，全面了解 nSi-MS 質譜檢測平台對小分子分析物的檢測能力以及其質譜檢測平台於生物醫學領域的未來發展方向和潛力。

關鍵字

前處理 ； 分離 ； 管制藥品 ； 奈米矽 ； 質譜

並列摘要

In this study, the nanostructured silicon (nSi) material was used as the mass spectrometry (MS) analysis substrate (nSi-MS). nSi-MS method especially suits for the detection of low m/z value analytes because of its’ matrix-free and high sensitivity characteristics; its’ simple pre-treatment and less sample consumption can also make analysis rapidly accomplished under 15 minute.
Urine specimen pre-treatment, peptides mixture separation and isoelectric focusing 2-D electrophoresis direct transfer had been proceeded to evaluate the specific pre-treatment performance of nSi-MS.
And we also applied nSi-MS to those small molecular abused drugs to realize the performance in low m/z value range. Standard drug samples and addict urine specimens were both investigated in this research, including amphetamine, methamphetamine, codeine, morphine, ketamine and MDEA; limit of detection, linearity, repeatability and quantitative analysis have been assayed and discussed in this document in order to fully understand the nSi-MS technique capability in small molecule detection and its’ future development potential in biomedical field.

並列關鍵字

separation ； pre-treatment ； abused drug ； nanostructured silicon ； mass spectrometry

參考文獻

[1] J. Wei, J. M. Buriak, and G. Siuzdak, "Desorption-ionization mass spectrometry on porous silicon," Nature, vol. 399, pp. 243-246, 1999.

[2] J. J. Thomas, Z. X. Shen, R. Blackledge, and G. Siuzdak, "Desorption-ionization on silicon mass spectrometry: an application in forensics," Analytica Chimica Acta, vol. 442, pp. 183-190, 2001.

[3] K. Huikko, P. Ostman, C. Sauber, F. Mandel, K. Grigoras, S. Franssila, T. Kotiaho, and R. Kostiainen, "Feasibility of atmospheric pressure desorption/ionization on silicon mass spectrometry in analysis of drugs," Rapid Communications in Mass Spectrometry, vol. 17, pp. 1339-1343, 2003.

[4] K. Pihlainen, K. Grigoras, S. Franssila, R. Ketola, T. Kotiaho, and R. Kostiainen, "Analysis of amphetamines and fentanyls by atmospheric pressure desorption/ionization on silicon mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry and its application to forensic analysis of drug seizures," Journal of Mass Spectrometry, vol. 40, pp. 539-545, 2005.

[5] C. W. Tsao, P. Kumar, J. K. Liu, and L. Devoe, "Dynamic electrowetting on nanofilament silicon for matrix-free laser desorption/ionization mass spectrometry," Analytical Chemistry, vol. 80, pp. 2973-2981, 2008.