Preparation of Recombinant PP1 Binding Protein Complexes by Baculovirus Expression System

Preparation of Recombinant PP1 Binding Protein Complexes by Baculovirus Expression System

楊嘉倩(Jia-Qian Yang)

指導教授 ： 黃憲斌

國立中正大學/理學院/生命科學系分子生物研究所/碩士(2012年)

並列摘要

Protein phosphatase 1 (PP1) is one of the major cellular serine/threonine protein phosphatases in eukaryotic cells and participates in a wide range of cellular processes. Each functional PP1 holoenzyme consists of a catalytic subunit and a regulatory subunit. Most of the regulatory subunits contain a consensus Arg-Val-x-Phe (RVxF) sequence, which binds PP1 at a site distinct from the catalytic site.
Phostensin, C9orf75 and Hepp1 are the novel PP1 regulatory proteins and were identified by yeast two-hybrid screen in our laboratory. These three PP1 regulatory proteins exhibit different abilities in inhibition of PP1. Phostensin is more potent than C9orf75 in inhibition of PP1. On the other hand, Hepp1 cannot inhibit PP1 activity at the levels of μM. Structural basis for their differences in inhibition of PP1 is essential for understanding the mechanism in regulation of the enzymatic activity of PP1. In my study, I will prepare recombinant PP1 holoenzymes by baculovirus expression system for structural analysis.

並列關鍵字

PP1

參考文獻

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