Particle Plasmon Resonance Biosensor Based on Tubular Waveguide and Multi-window Optical Fiber for Multi-analyte Detection

Particle Plasmon Resonance Biosensor Based on Tubular Waveguide and Multi-window Optical Fiber for Multi-analyte Detection

劉又嘉(Liu, Yuchia)

指導教授 ： 周禮君

國立中正大學/理學院/化學暨生物化學系/碩士(2012年)

摘要

本論文之主要研究目的是利用光學方式發展出免標定、即時性並具有多工檢測能力的生化檢測系統。目前已建立兩種新穎式之多工光學生化檢測系統，分別為：多通道管狀光波導粒子電漿共振生物感測器(multi-channel TOW-PPR：Tubular optical waveguide particle plasmon resonance biosensor)、以及多視窗光纖粒子電漿共振生物感測器(MW-FOPPR：Multi-window fiber optic particle plasmon resonance biosensor)，並利用溶液折射率與生化結合反應等多種實驗測試進行系統的再現性(reproducibility)、穩定度(stability)、靈敏度(sensor sensitivity)與解析度(sensor resolution)之分析與評估，驗證系統之可行性。
此兩項系統皆是建立在貴金屬奈米粒子電漿共振（Particle Plasmon Resonance, PPR）效應作為感測的機制，利用光波導的原理將貴金屬奈米粒子修飾於波導基材表面，導入合適之粒子電漿共振波段之激發光源後，利用衰逝全反射方式耦合金奈米粒子產生粒子電漿共振，當樣品的折射率改變或是藉由貴金屬奈米粒子表面修飾上不同的生化分子，進行特異性吸附之生化反應後所產生的環境折射率變化時，電漿共振訊號將會隨之變化，藉由波導所造成的連續式多重全反射作用將光變化訊號進行增益後，利用量測穿透光強度變化達成偵測效果。
對於兩項系統皆進行一系列之折射率變化與生化檢測之實驗用以評估系統之穩定度、再現性、感測靈敏度、與感測解析度等特性。在折射率測試實驗中，利用不同重量比例的蔗糖與純水配置成不同折射率的蔗糖溶液，實驗結果顯示感測器解析度大約為2.7×10-5 RIU (refraction index unit)。在生化檢測實驗上，利用二硝基苯(DNP)對抗二硝基苯抗體(anti-DNP antibody)及維生素H (biotin) 對卵蛋白(streptavidin)的檢測，其檢量線之線性度>0.99，偵測極限<15.4pM。
本研究之兩種新穎性多工光學生化檢測系統不僅具有免標定與即時偵測的能力，並分別利用單一奈米粒子結合玻璃管完成多通道之平行多工檢測以及利用多視窗型光纖並在不同感測區域修飾具特殊相異電漿共振波段之奈米粒子完成多分析物之多工檢測分析。結果皆顯示其穩定度、再現性、感測靈敏度、與偵測極限等特性皆優異於現行之表面電漿共振或粒子電漿共振生化感測系統。

關鍵字

粒子電漿共振 ； 管狀光波導 ； 多視窗光纖 ； 多工檢測分析

並列摘要

The objective of this work is to develop two novel multiplex chemical and biochemical sensing platforms, namly multi-channel tubular optical waveguide particle plasmon resonance biosensor (multi-channel TOW-PPR ) and multi-window fiber optic particle plasmon resonance biosensor (MW-FOPPR), for real-time and label-free detection. The principle of the inventions are based on measuring the light intensity after consecutive total internal reflections (TIRs) along the noble metal nanoparticles-modified waveguide (tube and optical fiber), wherein the evanescent wave excites the particle plasmon resonance of the nanoparticles at the reflection interface. When a noble metal nanoparticle is influenced by the change of the refractive index of its surrounding environment, its particle plasmon resonance condition will change. This phenomenon can be used as the basis of chemical and biological sensing.
A variety of experiments were carried out to validate the stability, reproducibility, sensitivity, and refractive index resolution of the sensing platform. Using different weight percents of sucrose in pure water, the refractive index resolution of the sensor is found to be about 2.7 × 10-5 RIU. In the biochemical detection experiments, DNP and biotin were used to functionalize the gold nanoparticles in order to detect anti-DNP antibody and streptavidin, respectively. Results show that the calibration curves are linear (correlation coefficient >0.99) and the detection limits are less than 15.4pM.

並列關鍵字

particle plasmon resonance ； tubular optical waveguide ； multi-window fiber ； multi-analyte detection

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