- 學位論文
光學凝血檢測儀之晶片的開發研究
Development and Study of Bio-chip for Optical Coagulation Detector
摘要
現今微機電製程 (Micro-electro-mechanical system, MEMS)發展成熟，已被運用在機械、電子、光學、生醫檢測等方面。在生醫檢測儀器設備昂貴且須耗費大量樣本與時間，透過MEMS可將承載樣本的晶片縮小化，具備有微小化、快速檢測、減少樣本的消耗功用。 本研究利用PDMS與PMMA製作微流道，這兩種材料常被使用在生醫檢測上，具有便宜、良好的透光度與生物相容性。透過MEMS製程的微流道利用表面張力驅動，並設計一生物晶片，並探討血液在這兩種材料上的接觸角變化。再將血液與凝劑以一定的比例混合後，血液會產生纖維蛋白凝塊與時間變化，並在37度恆溫室使用我們自製的檢測儀器所測得的訊號並且能夠在臨床上的使用。 最後結果顯示，在尺寸設計上PMMA流道長寬高的設計尺寸為10 mm×6.5 mm×300 μm，可搭配我們自製檢測儀器使用並在37度恆溫室具有良好的填充效果。在40次的實驗，PMMA流道對Glass流道相關係數 (R)為r=0.957，對手撈法與醫院用大型儀器 (ACL TOP)測得的凝血原酶時間相關係數 (R)為r=0.992 與r=0.912，可得知PMMA流道能與自製檢測儀器搭配，並在使用上也方便比較符合消費者使用。研究中也進行血液細胞與接觸角的關係，統計結果顯示出Hb、Hct、MCHC (P<0.05)對接觸角會有顯著的影響，並造成填充不易的情形。
並列摘要
Nowadays, the development of Micro-electro-mechanical system (MEMS) is mature, which has been used in machinery, electronics, optics, biomedical examinations and other fields. Through MEMS, wafer of bearing samples with the function of miniaturization, rapid inspection and reduction of sample consumption, can be downsized. Then to deal with the high expense of biomedical test equipment and requirement of large samples and time. In this study, PDMS and PMMA are used to produce microchannel. These two materials are commonly used in biomedical testing, and has a cheap, good transmittance and biocompatibility. Through the microchannel in production process of MEMS driven by surface tension, and designed a biochip, then explore the change of contact angle in blood on each two materials. Then, we mixed the blood and thromboplastin in a certain ratio. Then, the blood will lead to fibrin clot and timing change, which can be used via temperature-controlled chamber at 37 ° C and using our self-made detection equipment to the measured signal and can be used in clinical. Final result shows that the size of length, width and height of PMMA flow channel on the design dimensions of 10 mm × 6.5 mm × 300 μm, which can be used with our self-made detection equipment and has good filling effect at thermostatic chamber at 37 ° C. In 40 experiments, the correlated coefficient (R) of PMMA flow channel to Glass is r=0.957. The correlated coefficient is 0.992 between the PMMA flow channel and manual method. The correlated coefficient is 0.912 between the PMMA flow channel and hospital blood analyzer (ACL TOP). As the result, we can infer that PMMA flow channel and self-made detection equipment can used together, and more convenient for consumers. In this study, we also explored the relation between blood cells and contact angle. Statistical results shows that Hb, Hct, MCHC (P <0.05) have an significance on the contact angle and cause filling difficult situation.